Understanding and prevention of the fibrotic process in denervatedrat skeletal muscle

2009 Fiscal Year Final Research Report

• Project/Area Number: 19700458
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Hiroshima International University
• Principal Investigator: OZAWA Junya Hiroshima International University, 保健医療学部・理学療法学科, 講師 (00435059)
• Project Period (FY): 2007 – 2009
• Keywords: 線維化 / TGF-ss1 / 脱神経 / 骨格筋 / collagen

Research Abstract

In the soleus muscle, type I and III collagen deposition increased at intramusclular spaces following 7 and 14 days of denervation. Realtime PCR analysis revealed that type I collagen (COL1A1) mRNA expression did not change, and type III collagen (COL3A1) transiently decreased at 3 days after denervation. TGF-ss1 mRNA and protein expressions, on the other hand, elevated in a time-dependent manner, both TGF-ss1 mRNA and protein levels significantly increased at 14 days of denervation. TypeI and III collagen were increased with TGF-ss1 synchronously in denervated muscles in the early stage of fibrotic process.