2009 Fiscal Year Final Research Report
Investigation of the mechanism of heme degradation by heme oxygenase-1
| Project/Area Number |
19750150
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kurume University |
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Principal Investigator |
SATO Hideaki Kurume University, 医学部, 准教授 (60271996)
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| Project Period (FY) |
2007 – 2009
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| Keywords | ヘム代謝 / 酵素反応機構 / 酸化還元電位 / 構造生物学 |
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| Research Abstract |
Heme oxygenase (HO) catalyses the degradation of heme via three successive monooxygenase reactions. To probe the cleavage mechanism of oxaporphyrin ring of ferrous a-verdoheme, an intermediate in the HO reaction, ferrous verdoheme-rat HO-1 complex was prepared under anaerobic conditions. Electrochemical reduction of the complex was performed under anaerobic conditions and the reduction potential for one-electron reduction of the oxaporphyrin ring of ferrous verdoheme was determined. Effects of ligands such as CO on the electrochemical reduction and the electron transfer from NADPH-cytochrome P450 reductase to the complex were investigated. In addition, the crystal structure of rat HO-1 in complex with ferrous a -verdoheme was determined. On the basis of results in these experiments, the degradation mechanism of ferrous verdoheme under the physiological condition has discussed.
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[Presentation] Ferrousα-Verdoheme in Complex with Rat Heme Oxygenase-12009
Author(s)
Hideaki Sato, Masakazu Sugishima, Yuichiro Higashimoto, Hiroshi Sakamoto, Chizu Shimokawa, Masato Noguchi
Organizer
Its Crystal Structure and Electrochemistry (The 6th International Congress on Heme Oxygenases: Heme Oxygenases in Biology and Medicine
Place of Presentation
Miami Beach, Florida, U.S.A.
Year and Date
2009-10-02
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