Allograft rejection : Study on novel receptors on innate immune cells for the allo-MHCs.

2010 Fiscal Year Final Research Report

• Project/Area Number: 19770117
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Osaka Medical College
• Principal Investigator: YAMAJI Junko (田代 純子) Osaka Medical College, 医学部, 講師 (40340559)
• Research Collaborator: SHIMUZU Tetsunosuke 大阪医科大学, 消化器外科学, 大学院生 ; INOUE Yoshihiro 大阪医科大学, 消化器外科学, 大学院生 ; YAMANA Hidenori 大阪医科大学, 消化器外科学, 大学院生
• Project Period (FY): 2007 – 2010
• Keywords: 免疫学 / 移植・再生医療 / 遺伝子 / 細胞・組織 / 生体分子

Research Abstract

The purpose of this study is to investigate the molecular mechanism and the physiological role of the macrophage MHC receptor (MMR) 1 and MMR2. The gene for MMR protein was found to be conserved in various animals and human, and we succeeded in the isolation of cDNAs encoding the human homologues of mouse MMR1 and MMR2. We found a rule for the expression of mouse MMRs on PBMCs : they express MMRs for nonself MHCs, implying that MMRs might play a physiological important role in allorecognition.

Research Products

• [Journal Article] Transgene Number-dependent, Gene Expression Rate-independent Rejection of D^d-, K^d-, or D^dK^d-Transgened Mouse Skin or Tumor Cells from C57BL/6 (DbKb)Mice. (2011)
  • Author(s): Yoshihiro Inoue, Junko Tashiro-Yamaji, Michihiro Hayashi, Hiroshi Kiyonari, Tetsunosuke Shimizu, Minenori Ibata, Hidenori Yamana, Takahiro Kubota, Nobuhiko Tanigawa, Ryotaro Yoshida.
  • Journal Title: Microbiology and Immunology in press
  • Peer Reviewed
• [Journal Article] HLA-B 62 as a possible ligand for the human homologue of mouse macrophage MHC receptor 2 (MMR 2) on monocytes. (2010)
  • Author(s): Tetsunosuke Shimizu, Junko Tashiro-Yamaji, Michihiro Hayashi, Yoshihiro Inoue, Minenori Ibata, Takahiro Kubota, Nobuhiko Tanigawa, Ryotaro Yoshida.
  • Journal Title: Gene. 454(1-2) Pages: 31-38
  • Peer Reviewed
• [Presentation] Isolation of a human cDNA homologous to a mouse cDNA encoding a receptor for allogeneic MHC (H-2 D^d)[macrophage MHC receptor 1 (MMR 1)] (2010)
  • Author(s): Junko Tashiro-Yamaji, Tetsunosuke Shimizu, Yoshihiro Inoue, Takahiro Kubota, Ryotaro Yoshida.
  • Organizer: 1 4 ^<th>International Congress of Immunology(第14回国際免疫学会)
  • Place of Presentation: 神戸市・Kobe International Exhibition Hall.
  • Year and Date: 2010-08-25
• [Presentation] Isolation of a human cDNA homologous to a mouse cDNA encoding a receptor for allogeneic MHC (H-2D^d) [macrophage MHC receptor 1 (MMR1)] (2010)
  • Author(s): Junko Tashiro-Yamaji, Tetsunosuke Shimizu, Hidenori Yamana, Wakako Miyachi, Keiko Hasegawa, Yukako Shiraiwa, Yoshiaki Mori, Ryotaro Yoshida, Takahiro Kubota.
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市・盛岡市民文化ホール(マリオス)
  • Year and Date: 2010-05-19
• [Presentation] Self/nonself recognition : HLA-B61 and HLA-B62 as possible ligands for the human homologue of mouse MHC receptor 2(MMR2) (2009)
  • Author(s): Tetsunosuke Shimizu, Junko Tashiro-Yamaji, Nobuhiko Tanigawa, Minenori Ibata, Michihiro Hayashi, Yoshihiro Inoue, Takahiro Kubota, Ryotaro Yoshida.
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートピアホテル(兵庫県神戸市)
  • Year and Date: 2009-10-24
• [Presentation] Isolation and characterization of a cDNA encoding a novel MHC receptor, macrophage MHC receptor(MMR) 2, in mouse and human. (2009)
  • Author(s): Junko Tashiro-Yamaji, Ryotaro Yoshida, Tetsunosuke Shimizu, Michihiro Hayashi, Yoshihiro Inoue, Minenori Ibata, Hiromi Nakanishi, Yoshiaki Mori, Nobuhiko Tanigawa, Takahiro Kubota.
  • Organizer: 第36回国際生理学会(IUPS2009)
  • Place of Presentation: 国立京都国際会館(京都府京都市)
  • Year and Date: 2009-07-31
• [Presentation] Self/nonself recognition : Isolation and expression of a cDNA encoding a novel human receptor, which recognizes HLA-B15, homologous to mouse macrophage MHC receptor 2(MMR2) (2008)
  • Author(s): Tetsunosuke Shimizu, Junko Tashiro-Yamaji, Nobuhiko Tanigawa, Minenori Ibata, Michihiro Hayashi, Yoshihiro Inoue, Takahiro Kubota, Ryotaro Yoshida.
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県神戸市)
  • Year and Date: 20081211-20081212
• [Presentation] Mice lacking macrophage MHC(H-2Kd) receptor 2、3学会合同集会 (2008)
  • Author(s): 山路純子(田代純子)、伊川正人、岡部勝、清水徹之介、井畑峰紀、井上善博、平野雅予、森禎章、窪田隆裕、吉田龍太郎
  • Organizer: 第73回日本インターフェロン・サイトカイン学会学術集会/第19回日本生体防御学会学術総会/第45回補体シンポジウム
  • Place of Presentation: 北海道大学学術交流会館(北海道札幌市)
  • Year and Date: 2008-07-11
• [Presentation] Screening of mouse embryonic stem (ES) cells lacking macrophage MHC (H-2Kd) receptor 2 (2008)
  • Author(s): 山路純子(田代純子)、吉田龍太郎、伊川正人、岡部勝、清水徹之介、井畑峰紀、平野雅予、森禎章、窪田隆裕
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 京王プラザホテル東京(東京都新宿区)
  • Year and Date: 2008-03-27
• [Presentation] Isolation of a human cDNA homologous to murine macrophage MHC receptor 1 (MMR-1) (2007)
  • Author(s): 清水徹之介、山路純子(田代純子)、谷川允彦, 井畑峰紀, 林道廣, 井上善博, 窪田隆裕, 吉田龍太郎
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2007-12-12
• [Remarks] ホームページ等