2010 Fiscal Year Final Research Report
A study of gene targeting method in amphibians
| Project/Area Number |
19770191
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ONUMA Yasuko The University of Tokyo, 幹細胞工学研究センター, 研究員 (90431824)
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| Project Period (FY) |
2007 – 2010
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| Keywords | 発生・分化 / 動物 / 遺伝学 / 細胞・組織 / 両生類 |
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| Research Abstract |
The main aim is establishment of a pluripotent stem cell line as a basic technology for gene targeting in amphibians. For establishment of embryonic germ (EG) cell line, the tissue piece including fluorescent-marked primordial germ cells (PGC) was isolated by manipulation from embryos and was preferred to examine culture condition. Using early embryos and primary cell culture, gene-transfer and culture methods were investigated to produce induced pluripotent stem cell (iPS cell).
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