2010 Fiscal Year Final Research Report
In vitro assessment of three-dimensional tissue engineered articular cartilage with a combination of quantitative MR imaging techniques
| Project/Area Number |
19790896
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | Teikyo University |
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Principal Investigator |
WATANABE Atsuya Teikyo University, 医学部, 准教授 (40375712)
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| Project Period (FY) |
2007 – 2010
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| Keywords | 再生軟骨 / 間葉系幹細胞 / 三次元培養軟骨 / 磁気共鳴撮像 |
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| Research Abstract |
In this study, we established an in vitro quantitative MR imaging technique of 3D tissue engineered cartilage with a combination of dGEMRIC, T2 mapping, and 3D SPGR MRI. Using the established MR imaging technique, we determined the optimal conditions for culturing 3D tissue engineered cartilage using rabbit mesenchymal stem cell, including seeding cell number, scaffold size, and in vitro culture duration.
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