Analysis of intercellular signaling molecules working in organ development and differentiation in plants

2011 Fiscal Year Final Research Report

• Project/Area Number: 19GS0315
• Research Category: Grant-in-Aid for Creative Scientific Research
• Allocation Type: Single-year Grants
• Research Institution: National Institute for Basic Biology
• Principal Investigator: OKADA Kiyotaka 基礎生物学研究所, 所長 (50101093)
• Co-Investigator(Kenkyū-buntansha): TSUGEKI Ryuji 京都大学, 理学研究科, 助教 (50303805) ; SAWA Shin-ichiro 熊本大学, 自然科学研究科, 教授 (00315748) ; MATSUBAYASHI Yoshikatsu 基礎生物学研究所, 細胞間シグナル研究部門, 教授 (00313974) ; TATEMATS Kiyoshi 基礎生物学研究所, 植物器官形成学研究室, 助教 (00373324)
• Project Period (FY): 2007 – 2011
• Keywords: 植物発生 / 葉 / 表裏 / アポプラスト / マイクロRNA / 茎頂分裂組織 / ペプチドシグナル

Research Abstract

In order to examine intercellular signaling systems working in organ formation and development, we took several approaches. We identified small LTP proteins in apoplastic extracts of cauliflower heads having a role of formation and maintenance of shoot apical meristem. Genetic approach revealed that trimeric G-protein is involved in the intracellular signing pathway under the peptide hormone receptors that regulate shoot and root meristem function. We showed novel group of tyrosine-sulfated glycopeptides had important roles as intercellular signals in plant development. We investigated action of microRNA165/166 in forming the boundary between the adaxial- and the abaxial-regions in developing leaves. In addition, we developed instrumental systems inducing specific gene(s) in a single plant cell or inactivating a single cell by use of laser beams

Research Products

• [Journal Article] The leaf adaxial-abaxial boundary and lamina growth. Plants (2013)
  • Author(s): Nakata, M. & Okada, K
  • Journal Title: Plos Genetics Volume: (in press)
  • Peer Reviewed
• [Journal Article] Pattern Dynamics in Adaxial-Abaxial Specific Gene Expression is Modulated by Plastid Retrograde Signal during Arabidopsis leaf development (2013)
  • Author(s): Tameshige, T., Fujita, H., Watanabe, K., Toyokura, K., Kondo, M., Tatematsu, K., Matsumoto, N., Tsugeki, R., Kawaguchi, M., Nishimura, M., Okada. K
  • Journal Title: Plos Genetics
  • Peer Reviewed
• [Journal Article] Differential Effects of Peptides Stomagen, EPF1, and EPF2 on Activation of the MAP Kinase MPK6 and the SPCH Protein Level (2013)
  • Author(s): Jewaria, P. K., Hara, T., Tanaka, H., Kondo, T., Betsuyaku, S., Sawa, S., Sakagami, Y., Aimoto S. and Kakimoto, T
  • Journal Title: Plant Cell Physiol
  • Peer Reviewed
• [Journal Article] CLE peptide function in the SAM and CLE peptide evolution in the land plant (2013)
  • Author(s): Miyawaki, K., Tabata, R., and Sawa, S
  • Journal Title: Curr. Opin. Plant Biol
  • Peer Reviewed
• [Journal Article] Tatematsu, K., Okada, K.: Physical interaction of floral organs controls petal morphogenesis in Arabidopsis thaliana (2013)
  • Author(s): Takeda, S., Iwasaki, A., Matsumoto, N., Uemura, T
  • Journal Title: Plant Physiol
  • Peer Reviewed
• [Journal Article] Chemical synthesis of Arabidopsis CLV3 glycopeptide reveals the impact of hydroxyproline arabinosylation on peptide conformation and activity (2013)
  • Author(s): Shinohara, H., Matsubayashi, Y
  • Journal Title: Plant Cell Physiol Volume: 54 Pages: 369-374
  • Peer Reviewed
• [Journal Article] Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis (2012)
  • Author(s): Nakata, M., Matsumoto, N., Tsugeki, R., Rikirsch, E., Laux, T., Okada, K
  • Journal Title: Plant Cell Volume: 24 Pages: 519-535
  • Peer Reviewed
• [Journal Article] The function of CLE and other plant peptide hormones in root development (2012)
  • Author(s): Yamada, M., and Sawa, S
  • Journal Title: Curr. Opin Volume: Plant Biol16 Pages: 1-6
  • Peer Reviewed
• [Journal Article] The role of CLE peptides in Arabidopsis development and nematode parasitism (2012)
  • Author(s): Kiyohara, S., and Sawa, S
  • Journal Title: Plant Cell Physiol Volume: 53 Pages: 1989-1999
  • Peer Reviewed
• [Journal Article] SNPs of CLAVATA r eceptors in tomato, in a context of rootknot nematode infection (2012)
  • Author(s): Ejima, C, Uwatoko, T, Ngan, B. T., Honda, H., Shimizu, N., Kiyohara, S., Hamasaki, R., and Sawa, S
  • Journal Title: Nematological Research Volume: 2 Pages: 35-40
  • Peer Reviewed
• [Journal Article] Biochemical mapping of a ligand-binding domain within Arabidopsis BAM1 reveals diversified ligand recognition mechanisms of plant LRR-RKs (2012)
  • Author(s): Shinohara, H., Moriyama, Y., Ohyama, K., Matsubayashi Y
  • Journal Title: Plant J Volume: 70 Pages: 845-854
  • Peer Reviewed
• [Journal Article] Succinic Semialdehyde Dehydrogenase is Involved in the Robust Patterning of Arabidopsis Leaves along the Adaxial-Abaxial Axis (2011)
  • Author(s): Toyokura, K., Watanabe, K., Oiwaka, A., Kusano, M., Tameshige, T., Tatematsu, K., Matsumoto, N., Tsugeki, R., Saito, K., Okada, K
  • Journal Title: Plant & Cell Physiology Volume: 52 Pages: 1340-1353
  • Peer Reviewed
• [Journal Article] Secreted peptide signals required for maintenance of root stem cell niche in Arabidopsis (2010)
  • Author(s): Matsuzaki Y., Ogawa-Ohnishi M., Mori A., Matsubayashi Y
  • Journal Title: Science Volume: 329 Pages: 1065-1067
  • Peer Reviewed
• [Journal Article] Jasmonic acid control of GLABA3 links inducible defense and trichome patterning in Arabidopsis (2009)
  • Author(s): Yoshida, Y., Sano, R., Wada, T., Takabayashi, J., Okada, K
  • Journal Title: Development Volume: 136 Pages: 1039-1048
  • Peer Reviewed
• [Journal Article] The Novel Nuclear Factor NO VEIN Mediates Auxin-Dependent Specification and Patterning in the Embryo, Shoot and Root (2009)
  • Author(s): Tsugeki, R., Ditengou, F. A., Sumi, Y., Palme, K., Okada. K
  • Journal Title: Plant Cell Volume: 21 Pages: 3133-3151
  • Peer Reviewed
• [Journal Article] Identification of tyrosylprotein sulfotransferase in Arabidopsis (2009)
  • Author(s): Komori, R., Amano, Y., Ogawa-Ohnishi, M. and Matsubayashi, Y
  • Journal Title: Proc Natl Acad Sci U S A Volume: 106 Pages: 15067-1507215067-15072
  • Peer Reviewed
• [Journal Article] A glycopeptide regulating stem cell fate in Arabidopsis thaliana (2009)
  • Author(s): Ohyama, K., Shinohara, H., Ogawa-Ohnishi, M. and Matsubayashi, Y
  • Journal Title: Nature Chem Biol Volume: 5 Pages: 578-580
  • Peer Reviewed
• [Journal Article] and Wasteneys, G.: Armadillo repeat-containing kinesins and a NIMA-related kinase are required for epidermal cell morphogenesis in Arabidopsis (2008)
  • Author(s): Sakai, T., van der Honing, H., Nishioka, M., Uehara, Y., Takahashi, M., Fujisawa, N., Saji, K., Seki, M., Shinozaki, K., Jones, M., Smirnoff, N., Okada, K
  • Journal Title: Plant J Volume: 53 Pages: 157-171
  • Peer Reviewed
• [Journal Article] Arabidopsis CLV3 peptide directly binds CLV1 ectodomain (2008)
  • Author(s): Ogawa, M., Shinohara, H., Sakagami, Y. and Matsubayashi, Y
  • Journal Title: Science
  • Peer Reviewed
• [Journal Article] Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system. (2008)
  • Author(s): Hirakawa, Y., Shinohara, H., Kondo, Y., Inoue, A., Nakanomyo, I., Ogawa, M., Sawa, S., Ohashi-Ito, K., Matsubayashi, Y. and Fukuda, H
  • Journal Title: Proc Natl Acad Sci Volume: 105 Pages: 15208-15213
  • Peer Reviewed
• [Journal Article] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis (2008)
  • Author(s): Ohyama, K., Ogawa, M. and Matsubayashi, Y
  • Journal Title: Plant J Volume: 55 Pages: 152-160
  • Peer Reviewed
• [Journal Article] Tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis (2007)
  • Author(s): Amano, Y., Tsubouchi, H., Shinohara, H., Ogawa, M. and Matsubayashi, Y
  • Journal Title: Proc Natl Acad Sci Volume: 104 Pages: 18333-18338
  • Peer Reviewed
• [Journal Article] Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling (2007)
  • Author(s): Shinohara, H., Ogawa, M., Sakagami, Y. and Matsubayashi, Y
  • Journal Title: J Biol Chem Volume: 282 Pages: 124-131
  • Peer Reviewed
• [Presentation] Secreted peptide signals required for maintenance of root stem cell niche in Arabidopsis. The 3rd NIBB-TLL-MPIZ Joint Symposium 2011 "Cell Cycle and Development" (2011)
  • Author(s): Matsubayashi Y
  • Organizer: Temasek Life Sciences Laboratory
  • Place of Presentation: Singapore
  • Year and Date: 20111121-22
• [Presentation] CLVATA signaling in meristem maintenance (2010)
  • Author(s): Sawa, S
  • Organizer: Institute seminar, Temasek Life Sciences Laboratory
  • Place of Presentation: Singapore
  • Year and Date: 20100524-26
• [Presentation] Physiological importance of post-translational modifications in peptide hormone signaling (2010)
  • Author(s): Matsubayashi, Y
  • Organizer: 20th International Conference on Plant Growth Substances
  • Place of Presentation: Tarragona, Spain
  • Year and Date: 2010-07-28
• [Presentation] The abaxial-side specific expression of MIR165/166 clearly marks off the PHB-expression domain from the FIL-expression domain in Arabidopsis leaf primordia (2009)
  • Author(s): Tatematsu, K., Watanabe, K., Toyokura, K., Tameshige, T., and Okada, K
  • Organizer: The 20th International Conference on Arabidopsis Research
  • Place of Presentation: Edinburgh, Scotland
  • Year and Date: 20090630-0704
• [Presentation] CLE peptide function in meristem size maintenance in Arabidopsis (2009)
  • Author(s): Sawa, S
  • Organizer: Faculty seminar, Seoul National University
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2009-09-18
• [Presentation] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis (2008)
  • Author(s): Matsubayashi, Y., Ohyama, K., Ogawa, M
  • Organizer: Japan-Korea Symposium - Plant Growth and Signal Transduction
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20080609-10
• [Presentation] Region-specific expresson of regulatory genes in the lateral organ formation (2008)
  • Author(s): Okada, K
  • Organizer: SigNet International Symposium 2008 Korea University
  • Place of Presentation: Korea
  • Year and Date: 20080220-21
• [Presentation] CLE peptide function in meristem size maintenance in Arabidopsis (2008)
  • Author(s): Sawa, S
  • Organizer: Institute seminar; University of Duesseldorf
  • Place of Presentation: Germany
  • Year and Date: 2008-05-06
• [Presentation] Roles of two structurally distinct sulfated peptides, PSK and PSY1, in Arabidopsis growth and development (2007)
  • Author(s): Matsubayashi Y
  • Organizer: 19th International Conference on Plant Growth Substances
  • Place of Presentation: Puerto Vallarta, Mexico
  • Year and Date: 20070721-25
• [Presentation] Axis-dependent gene expression in the lateral organ formation (2007)
  • Author(s): Okada, K
  • Organizer: The 18th International Conference on Arabidopsis Research, Beijing
  • Place of Presentation: China
  • Year and Date: 20070620-23
• [Presentation] Physiological functions of two structurally distinct tyrosine-sulfated peptides in Arabidopsis growth and development (2007)
  • Author(s): Matsubayashi Y
  • Organizer: 18th International Conference on Arabidopsis Research
  • Place of Presentation: China
  • Year and Date: 20070620-23
• [Book] The Arabidopsis (2011)
  • Author(s): Matsubayashi, Y
  • Publisher: Small post-translationally modified peptide signals in Arabidopsis
• [Book] 新しい植物ホルモンの科学 第2版 (2010)
  • Author(s): 松林嘉克
  • Publisher: 講談社
• [Book] オーキシン、新しい植物ホルモンの科学、第2版、小柴、神谷編 (2007)
  • Author(s): 小柴共一、澤 進一郎
  • Publisher: 講談社
• [Remarks] 澤
  • URL: http://www.sci.kumamoto-u.ac.jp/~sawa/
• [Remarks] 松林
  • URL: http://www.nibb.ac.jp/ligand/
• [Remarks] 槻木
  • URL: http://shikanai.p1.bindsite.jp/research.html