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2011 Fiscal Year Final Research Report

Analysis of intercellular signaling molecules working in organ development and differentiation in plants

Research Project

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Project/Area Number 19GS0315
Research Category

Grant-in-Aid for Creative Scientific Research

Allocation TypeSingle-year Grants
Research InstitutionNational Institute for Basic Biology

Principal Investigator

OKADA Kiyotaka  基礎生物学研究所, 所長 (50101093)

Co-Investigator(Kenkyū-buntansha) TSUGEKI Ryuji  京都大学, 理学研究科, 助教 (50303805)
SAWA Shin-ichiro  熊本大学, 自然科学研究科, 教授 (00315748)
MATSUBAYASHI Yoshikatsu  基礎生物学研究所, 細胞間シグナル研究部門, 教授 (00313974)
TATEMATS Kiyoshi  基礎生物学研究所, 植物器官形成学研究室, 助教 (00373324)
Project Period (FY) 2007 – 2011
Keywords植物発生 / 葉 / 表裏 / アポプラスト / マイクロRNA / 茎頂分裂組織 / ペプチドシグナル
Research Abstract

In order to examine intercellular signaling systems working in organ formation and development, we took several approaches. We identified small LTP proteins in apoplastic extracts of cauliflower heads having a role of formation and maintenance of shoot apical meristem. Genetic approach revealed that trimeric G-protein is involved in the intracellular signing pathway under the peptide hormone receptors that regulate shoot and root meristem function. We showed novel group of tyrosine-sulfated glycopeptides had important roles as intercellular signals in plant development. We investigated action of microRNA165/166 in forming the boundary between the adaxial- and the abaxial-regions in developing leaves. In addition, we developed instrumental systems inducing specific gene(s) in a single plant cell or inactivating a single cell by use of laser beams

  • Research Products

    (40 results)

All 2013 2012 2011 2010 2009 2008 2007 Other

All Journal Article (23 results) (of which Peer Reviewed: 23 results) Presentation (11 results) Book (3 results) Remarks (3 results)

  • [Journal Article] The leaf adaxial-abaxial boundary and lamina growth. Plants2013

    • Author(s)
      Nakata, M. & Okada, K
    • Journal Title

      Plos Genetics

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Pattern Dynamics in Adaxial-Abaxial Specific Gene Expression is Modulated by Plastid Retrograde Signal during Arabidopsis leaf development2013

    • Author(s)
      Tameshige, T., Fujita, H., Watanabe, K., Toyokura, K., Kondo, M., Tatematsu, K., Matsumoto, N., Tsugeki, R., Kawaguchi, M., Nishimura, M., Okada. K
    • Journal Title

      Plos Genetics

    • Peer Reviewed
  • [Journal Article] Differential Effects of Peptides Stomagen, EPF1, and EPF2 on Activation of the MAP Kinase MPK6 and the SPCH Protein Level2013

    • Author(s)
      Jewaria, P. K., Hara, T., Tanaka, H., Kondo, T., Betsuyaku, S., Sawa, S., Sakagami, Y., Aimoto S. and Kakimoto, T
    • Journal Title

      Plant Cell Physiol

    • Peer Reviewed
  • [Journal Article] CLE peptide function in the SAM and CLE peptide evolution in the land plant2013

    • Author(s)
      Miyawaki, K., Tabata, R., and Sawa, S
    • Journal Title

      Curr. Opin. Plant Biol

    • Peer Reviewed
  • [Journal Article] Tatematsu, K., Okada, K.: Physical interaction of floral organs controls petal morphogenesis in Arabidopsis thaliana2013

    • Author(s)
      Takeda, S., Iwasaki, A., Matsumoto, N., Uemura, T
    • Journal Title

      Plant Physiol

    • Peer Reviewed
  • [Journal Article] Chemical synthesis of Arabidopsis CLV3 glycopeptide reveals the impact of hydroxyproline arabinosylation on peptide conformation and activity2013

    • Author(s)
      Shinohara, H., Matsubayashi, Y
    • Journal Title

      Plant Cell Physiol

      Volume: 54 Pages: 369-374

    • Peer Reviewed
  • [Journal Article] Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis2012

    • Author(s)
      Nakata, M., Matsumoto, N., Tsugeki, R., Rikirsch, E., Laux, T., Okada, K
    • Journal Title

      Plant Cell

      Volume: 24 Pages: 519-535

    • Peer Reviewed
  • [Journal Article] The function of CLE and other plant peptide hormones in root development2012

    • Author(s)
      Yamada, M., and Sawa, S
    • Journal Title

      Curr. Opin

      Volume: Plant Biol16 Pages: 1-6

    • Peer Reviewed
  • [Journal Article] The role of CLE peptides in Arabidopsis development and nematode parasitism2012

    • Author(s)
      Kiyohara, S., and Sawa, S
    • Journal Title

      Plant Cell Physiol

      Volume: 53 Pages: 1989-1999

    • Peer Reviewed
  • [Journal Article] SNPs of CLAVATA r eceptors in tomato, in a context of rootknot nematode infection2012

    • Author(s)
      Ejima, C, Uwatoko, T, Ngan, B. T., Honda, H., Shimizu, N., Kiyohara, S., Hamasaki, R., and Sawa, S
    • Journal Title

      Nematological Research

      Volume: 2 Pages: 35-40

    • Peer Reviewed
  • [Journal Article] Biochemical mapping of a ligand-binding domain within Arabidopsis BAM1 reveals diversified ligand recognition mechanisms of plant LRR-RKs2012

    • Author(s)
      Shinohara, H., Moriyama, Y., Ohyama, K., Matsubayashi Y
    • Journal Title

      Plant J

      Volume: 70 Pages: 845-854

    • Peer Reviewed
  • [Journal Article] Succinic Semialdehyde Dehydrogenase is Involved in the Robust Patterning of Arabidopsis Leaves along the Adaxial-Abaxial Axis2011

    • Author(s)
      Toyokura, K., Watanabe, K., Oiwaka, A., Kusano, M., Tameshige, T., Tatematsu, K., Matsumoto, N., Tsugeki, R., Saito, K., Okada, K
    • Journal Title

      Plant & Cell Physiology

      Volume: 52 Pages: 1340-1353

    • Peer Reviewed
  • [Journal Article] Secreted peptide signals required for maintenance of root stem cell niche in Arabidopsis2010

    • Author(s)
      Matsuzaki Y., Ogawa-Ohnishi M., Mori A., Matsubayashi Y
    • Journal Title

      Science

      Volume: 329 Pages: 1065-1067

    • Peer Reviewed
  • [Journal Article] Jasmonic acid control of GLABA3 links inducible defense and trichome patterning in Arabidopsis2009

    • Author(s)
      Yoshida, Y., Sano, R., Wada, T., Takabayashi, J., Okada, K
    • Journal Title

      Development

      Volume: 136 Pages: 1039-1048

    • Peer Reviewed
  • [Journal Article] The Novel Nuclear Factor NO VEIN Mediates Auxin-Dependent Specification and Patterning in the Embryo, Shoot and Root2009

    • Author(s)
      Tsugeki, R., Ditengou, F. A., Sumi, Y., Palme, K., Okada. K
    • Journal Title

      Plant Cell

      Volume: 21 Pages: 3133-3151

    • Peer Reviewed
  • [Journal Article] Identification of tyrosylprotein sulfotransferase in Arabidopsis2009

    • Author(s)
      Komori, R., Amano, Y., Ogawa-Ohnishi, M. and Matsubayashi, Y
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 106 Pages: 15067-1507215067-15072

    • Peer Reviewed
  • [Journal Article] A glycopeptide regulating stem cell fate in Arabidopsis thaliana2009

    • Author(s)
      Ohyama, K., Shinohara, H., Ogawa-Ohnishi, M. and Matsubayashi, Y
    • Journal Title

      Nature Chem Biol

      Volume: 5 Pages: 578-580

    • Peer Reviewed
  • [Journal Article] and Wasteneys, G.: Armadillo repeat-containing kinesins and a NIMA-related kinase are required for epidermal cell morphogenesis in Arabidopsis2008

    • Author(s)
      Sakai, T., van der Honing, H., Nishioka, M., Uehara, Y., Takahashi, M., Fujisawa, N., Saji, K., Seki, M., Shinozaki, K., Jones, M., Smirnoff, N., Okada, K
    • Journal Title

      Plant J

      Volume: 53 Pages: 157-171

    • Peer Reviewed
  • [Journal Article] Arabidopsis CLV3 peptide directly binds CLV1 ectodomain2008

    • Author(s)
      Ogawa, M., Shinohara, H., Sakagami, Y. and Matsubayashi, Y
    • Journal Title

      Science

    • Peer Reviewed
  • [Journal Article] Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system.2008

    • Author(s)
      Hirakawa, Y., Shinohara, H., Kondo, Y., Inoue, A., Nakanomyo, I., Ogawa, M., Sawa, S., Ohashi-Ito, K., Matsubayashi, Y. and Fukuda, H
    • Journal Title

      Proc Natl Acad Sci

      Volume: 105 Pages: 15208-15213

    • Peer Reviewed
  • [Journal Article] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis2008

    • Author(s)
      Ohyama, K., Ogawa, M. and Matsubayashi, Y
    • Journal Title

      Plant J

      Volume: 55 Pages: 152-160

    • Peer Reviewed
  • [Journal Article] Tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis2007

    • Author(s)
      Amano, Y., Tsubouchi, H., Shinohara, H., Ogawa, M. and Matsubayashi, Y
    • Journal Title

      Proc Natl Acad Sci

      Volume: 104 Pages: 18333-18338

    • Peer Reviewed
  • [Journal Article] Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling2007

    • Author(s)
      Shinohara, H., Ogawa, M., Sakagami, Y. and Matsubayashi, Y
    • Journal Title

      J Biol Chem

      Volume: 282 Pages: 124-131

    • Peer Reviewed
  • [Presentation] Secreted peptide signals required for maintenance of root stem cell niche in Arabidopsis. The 3rd NIBB-TLL-MPIZ Joint Symposium 2011 "Cell Cycle and Development"2011

    • Author(s)
      Matsubayashi Y
    • Organizer
      Temasek Life Sciences Laboratory
    • Place of Presentation
      Singapore
    • Year and Date
      20111121-22
  • [Presentation] CLVATA signaling in meristem maintenance2010

    • Author(s)
      Sawa, S
    • Organizer
      Institute seminar, Temasek Life Sciences Laboratory
    • Place of Presentation
      Singapore
    • Year and Date
      20100524-26
  • [Presentation] Physiological importance of post-translational modifications in peptide hormone signaling2010

    • Author(s)
      Matsubayashi, Y
    • Organizer
      20th International Conference on Plant Growth Substances
    • Place of Presentation
      Tarragona, Spain
    • Year and Date
      2010-07-28
  • [Presentation] The abaxial-side specific expression of MIR165/166 clearly marks off the PHB-expression domain from the FIL-expression domain in Arabidopsis leaf primordia2009

    • Author(s)
      Tatematsu, K., Watanabe, K., Toyokura, K., Tameshige, T., and Okada, K
    • Organizer
      The 20th International Conference on Arabidopsis Research
    • Place of Presentation
      Edinburgh, Scotland
    • Year and Date
      20090630-0704
  • [Presentation] CLE peptide function in meristem size maintenance in Arabidopsis2009

    • Author(s)
      Sawa, S
    • Organizer
      Faculty seminar, Seoul National University
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2009-09-18
  • [Presentation] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis2008

    • Author(s)
      Matsubayashi, Y., Ohyama, K., Ogawa, M
    • Organizer
      Japan-Korea Symposium - Plant Growth and Signal Transduction
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      20080609-10
  • [Presentation] Region-specific expresson of regulatory genes in the lateral organ formation2008

    • Author(s)
      Okada, K
    • Organizer
      SigNet International Symposium 2008 Korea University
    • Place of Presentation
      Korea
    • Year and Date
      20080220-21
  • [Presentation] CLE peptide function in meristem size maintenance in Arabidopsis2008

    • Author(s)
      Sawa, S
    • Organizer
      Institute seminar; University of Duesseldorf
    • Place of Presentation
      Germany
    • Year and Date
      2008-05-06
  • [Presentation] Roles of two structurally distinct sulfated peptides, PSK and PSY1, in Arabidopsis growth and development2007

    • Author(s)
      Matsubayashi Y
    • Organizer
      19th International Conference on Plant Growth Substances
    • Place of Presentation
      Puerto Vallarta, Mexico
    • Year and Date
      20070721-25
  • [Presentation] Axis-dependent gene expression in the lateral organ formation2007

    • Author(s)
      Okada, K
    • Organizer
      The 18th International Conference on Arabidopsis Research, Beijing
    • Place of Presentation
      China
    • Year and Date
      20070620-23
  • [Presentation] Physiological functions of two structurally distinct tyrosine-sulfated peptides in Arabidopsis growth and development2007

    • Author(s)
      Matsubayashi Y
    • Organizer
      18th International Conference on Arabidopsis Research
    • Place of Presentation
      China
    • Year and Date
      20070620-23
  • [Book] The Arabidopsis2011

    • Author(s)
      Matsubayashi, Y
    • Publisher
      Small post-translationally modified peptide signals in Arabidopsis
  • [Book] 新しい植物ホルモンの科学 第2版2010

    • Author(s)
      松林嘉克
    • Publisher
      講談社
  • [Book] オーキシン、新しい植物ホルモンの科学、第2版、小柴、神谷編2007

    • Author(s)
      小柴共一、澤 進一郎
    • Publisher
      講談社
  • [Remarks] 澤

    • URL

      http://www.sci.kumamoto-u.ac.jp/~sawa/

  • [Remarks] 松林

    • URL

      http://www.nibb.ac.jp/ligand/

  • [Remarks] 槻木

    • URL

      http://shikanai.p1.bindsite.jp/research.html

URL: 

Published: 2014-08-29  

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