Single Molecule Mechanics and Computer Modeling of Cascading System of Biological Information Transfer

2011 Fiscal Year Final Research Report

• Project/Area Number: 19GS0418
• Research Category: Grant-in-Aid for Creative Scientific Research
• Allocation Type: Single-year Grants
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: IKAI Atsushi 東京工業大学, イノベーション研究推進体, 特任教授 (50011713)
• Co-Investigator(Kenkyū-buntansha): KISHIMOTO Kikuo 東京工業大学, 大学院・理工学研究科, 教授 (30111652) ; OSADA Toshiya 東京工業大学, 大学院・生命理工学研究科, 准教授 (00201997) ; OHTANI Hiroyuki 東京工業大学, 大学院・生命理工学研究科, 准教授 (80203826) ; AFRIN Rehana 東京工業大学, バイオフロンティアセンター, 特任准教授 (70447556) ; TAGAMI Katsunor 早稲田大学, 特任講師 (40361571) ; SEKIGUCHI Hiroshi 東京工業大学, 大学院・生命理工学研究科, 助教 (00401563)
• Project Period (FY): 2007 – 2011
• Keywords: 原子間力顕微鏡 / 細胞骨格 / ネットワーク力学 / 分子間相互作用力 / ストレスファイバー / アクチン

Research Abstract

The aim of this project is to perform mechanically quantitative experiments to clarify the mechanical and wound healing characteristics of the cytoskeletal and contribute to the single cell manipulations and surgery. We used a newly developed nano-mechanical methods to measure the different mechanical properties of the red blood cell and fibroblast(especially those of actin stress fibers) and constructed mechanical models to simulate the results. We obtained solid mechanical evidence that showed strong association of stress fibers with cortical actomyosin layer. This association forms the basis for the cytoskeleton and cell membrane cooperation during cell locomotion and information transfer.

Research Products

• [Journal Article] Forced Extension of delipidated red blood cell cytoskeleton with little indication of spectrin unfolding (2012)
  • Author(s): Afrin R, Nakaji M, Sekiguchi H, Lee D, Kishimoto K, Ikai A
  • Journal Title: Cytoskeleton Volume: 69巻 Pages: 101-112
  • DOI: doi:10.1002/cm.21001
  • Peer Reviewed
• [Journal Article] Fabricated cantilever for AFM measurements and manipulations (2012)
  • Author(s): Shinichi Machida, Takahiro Watanabe-Nakayama, Masakazu Saito, Rehana Afrin, and Atsushi Ikai
  • Journal Title: Pre-stress analysis of stress fibers, Micron Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Nonlinear displacement of ventral stress fibers under externally applied lateral force by an atomic force microscope (2011)
  • Author(s): Hakari T, Sekiguchi H, Osada T, Kishimoto K, Afrin R, Ikai A
  • Journal Title: Cytoskeleton Volume: 68巻 Pages: 628-638
  • DOI: doi:10.1002/cm.20537
  • Peer Reviewed
• [Journal Article] Direct detection of cellular adaptation to local cyclic stretching at the single cell level by atomic force microscopy (2011)
  • Author(s): Watanabe-Nakayama T, Machida S, Harada I, Sekiguchi H, Afrin R, Ikai A
  • Journal Title: Biophysical Journal Volume: 100巻 Pages: 564-572
  • Peer Reviewed
• [Journal Article] Micro-scoop for Manipulation of Micro Objects (2010)
  • Author(s): Takahiro Watanabe-Nakayama, Shinichi Machida, Rehana Afrin, and Atsushi Ikai
  • Journal Title: Use of Fabricated Cantilever with Atomic Force Microscope、Small Volume: 6巻 Pages: 2853-2857
  • Peer Reviewed
• [Journal Article] Direct manipulation of intracellular stress fibres using a hook-shaped AFM probe (2010)
  • Author(s): Shinichi Machida, Takahiro Watanabe-Nakayama, Ichiro Harada, Rehana Afrin, Tomonobu Nakayama and Atsushi Ikai
  • Journal Title: Nanotechnology Volume: 21巻 Pages: 385102(1-6)
  • Peer Reviewed
• [Journal Article] Tensile Mechanics of Alanine Based Helical Polypeptide (2009)
  • Author(s): Rehana Afrin, Kazuki Shiga, Ichiro Takahashi and Atsushi Ikai
  • Journal Title: Force Spectroscopy vs. Computer Simulations, Biophys. J Volume: 96巻 Pages: 1105-1114
  • Peer Reviewed
• [Journal Article] Exploring Transferrin-Receptor Interactions at the Single-Molecule Level (2008)
  • Author(s): Alexandre Yersin、Toshiya Osada, Atsushi Ikai
  • Journal Title: Biophys. J Volume: 94巻 Pages: 230-240
  • Peer Reviewed
• [Presentation] Paxillin accumulation to a damaged site on a stress fiber (2012)
  • Author(s): Takahiro Watanabe-Nakayama, Masakazu Saito, Shin-ichi Machida, Rehana Afrin, and Atsushi Ikai
  • Organizer: Biophysical Society 56th Annual Meeting
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 20120225-29
• [Presentation] Exploring Atomic Force Microscopy for Nano-medical Applications (2012)
  • Author(s): Rehana Afrin, Takahiro Watanabe-Nakayama, Shinichi Machida, Masakazu, Saito and Atsushi Ikai
  • Organizer: ACMM22/ICONN2012/APMC10 Combined Conference
  • Place of Presentation: Perth, Australia
  • Year and Date: 20120205-09
• [Presentation] AFM for Cytoskeletal Mechanics (2012)
  • Author(s): Atsushi Ikai, Rehana Afrin, Shinichi Machida, Takahiro Watanabe-Nakayama, Masakazu, Saito and Kikuo Kishimoto
  • Organizer: Linz Winter Workshop 2012
  • Place of Presentation: Lintz, Austria
  • Year and Date: 20120204-07
• [Presentation] Manipulation of Individual Stress Fibers and Focal Adhesions with AFM to Understand the Basic Mechanics of the Cellular (2011)
  • Author(s): Atsushi Ikai, Rehana Afrin, Shinichi Machida, Takahiro Watanabe-Nakayama, Masakazu, Saito and Kikuo Kishimoto
  • Organizer: Construction, AFM for Bio Med, Paris
  • Place of Presentation: Paris, France
  • Year and Date: 20110823-27
• [Presentation] Linkage structures between ventral stress fibers revealed by atomic force and fluorescence microscopy、Seeing is Believing (2011)
  • Author(s): Atsushi Ikai, Rehana Afrin, Shinichi Machida, Takahiro Watanabe-Nakayama, Masakazu, Saito and Kikuo Kishimoto
  • Organizer: Imaging the Processes of Life
  • Place of Presentation: Santa Barbara, USA
  • Year and Date: 20110720-22
• [Presentation] Evaluation of the Interfacial Strength of Mutilyaered Materials by Peeling & Indentation Method (2011)
  • Author(s): Kikuo Kishimoto
  • Organizer: AUN/SEED-Net 3rd Regional Conference in Mechanical and Aerospace Technology
  • Place of Presentation: Manila, Phillipines
  • Year and Date: 20110304-05
• [Presentation] Mechanical Analysis of the Bio Cell Subjected to AFM Loading (2011)
  • Author(s): Kikuo Kishimoto, David Lee, Yotaro Shinosawa, Kazuaki Inaba, Rehana Afrin and Atsushi Ikai
  • Organizer: International Workshop on Biomedical Engineering & Biomechanics(invited)
  • Place of Presentation: 西安,中国
  • Year and Date: 2011-08-13
• [Presentation] Force Spectroscopy of Helical Polypeptides and Cytoskeleton Related Proteins Studied by Atomic Force Microscopy (2010)
  • Author(s): Atsushi Ikai, Rehana Afrin, Shin-ichi Machida and Takahiro Nakayama, David Lee and Kikuo Kishimoto
  • Organizer: The 17th International Microscopy Congress(IMC17)
  • Place of Presentation: Rio de Janeiro Brazil
  • Year and Date: 20100919-24
• [Presentation] Force Measurement and Intracellular Operation Using Customized AFM Cantilever、American (2010)
  • Author(s): S. Machida, T. W. Nakayama ; I. Harada ; R. Afrin ; T. Nakayama ; A. Ikai
  • Organizer: Biophysical Society 54th Annual Meeting
  • Place of Presentation: San Francisco USA
  • Year and Date: 20100220-24
• [Presentation] Direct Manipulation of Stress Fibers in the Cell Using Fabricated Atomic Force Microscopy Cantilevers、American Biophysical Society Symposium (2010)
  • Author(s): Shinichi Machida, Takahiro W. Nakayama, Ichiro Harada, Rehana Afrin, Tomonobu Nakayama, Atsushi Ikai
  • Organizer: Actin, the Cytoskeleton and the Nucleus
  • Place of Presentation: Singapore
  • Year and Date: 2010-11-09
• [Book] Eds. Anne-Sophie Duwez, Nicolas WilletMicroscopy (2012)
  • Author(s): Ikai, A., Afrin, R., Watanabe-Nakayama, T. and Machida, S
  • Total Pages: 287
  • Publisher: CRC Press、Molecular Manipulation with Atomic Force Microscopy
• [Book] Ed. Yves Dufrene (2012)
  • Author(s): Ikai, A., Afrin, R., Watanabe-Nakayama, T. and Machida, S
  • Total Pages: 460
  • Publisher: Pan Stanford Publishing、Life at the Nanoscale : Atomic Force Microscopy of Live Cells
• [Book] Eds. Arturo M. Baro, Ronald G. Reifenberger (2012)
  • Author(s): Ikai, A., Afrin, R., Watanabe-Nakayama, T., Machida, S. and Saito, M
  • Total Pages: 402
  • Publisher: Wiley-VCH Atomic Force Microscopy in Liquid
• [Book] Advances in Chemical Physics : Single Molecule Biophysics : Experiments and Theory(Eds : T. Komatsuzaki et al.) (2011)
  • Author(s): Atsushi Ikai Rehana Afrin Hiroshi Sekiguchi
  • Total Pages: 512
  • Publisher: Force to Unbind Ligand : Receptor Complexes and the Internal Rigidity of Globular Proteins Probed by Single-Molecule Force Spectroscopy
• [Book] Advances in biochemical engineering/biotechnology : Nano/Micro Biotechnology (2009)
  • Author(s): Atsushi Ikai
  • Total Pages: 270
  • Publisher: A Review on : Atomic Force Microscopy Applied to Nano-mechanics of the Cell
• [Book] Elsevierドイツ語版: Einfuhrung in Die Nanobiomechanik : Bildgebung Und Messung Durch Rasterkraftmikroskopie(German Edition) by Atsushi Ikai and Carsten Heinisch(Wiley-VCH Verlag GmbH) (2008)
  • Author(s): Atsushi Ikai
  • Publisher: The World of Nano-Biomechanics : Mechanical Imaging and Measurement by Atomic Force Microscopy
• [Remarks]
  • URL: http://www.ikai.bio.titech.ac.jp/