2022 Fiscal Year Final Research Report
Elucidation of malignant transformation based on epigenetic plasticity
| Project/Area Number |
19H01031
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Review Section |
Medium-sized Section 50:Oncology and related fields
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
ABURATANI Hiroyuki 東京大学, 先端科学技術研究センター, 特任研究員 (10202657)
|
|---|
| Project Period (FY) |
2019-04-01 – 2023-03-31
|
| Keywords | エピゲノム / 一細胞解析 / クロマチン |
|---|
| Outline of Final Research Achievements |
We identified a long noncoding (lnc) RNA involved in the regulation of Wnt signaling that is important for stem cell replication. This lncRNA is a direct target of β-catenin, is involved in the accessibility of the transcriptional regulatory regions of a subset of CTNNB1 targets, such as LGR5 and ASCL2, where additional transcription factor seems to bind and co-operate with CTNNB1. On the other hand, single-cell transcriptome and chromatin analyzes of reproliferating cell populations after anticancer drug treatment identified pioneer factor candidates that bind to regulatory regions that are activated prior to proliferation.
|
| Free Research Field |
ゲノム科学
|
| Academic Significance and Societal Importance of the Research Achievements |
抗がん剤治療により増殖を停止した状態でパーシスターとして残存する腫瘍細胞のコントロールはがん治療の大きな課題である。本課題において同定された幹細胞増殖に関与する新規lncRNAおよびCTNNB1と協調する転写因子、再増殖に先だって活性化されるパイオニア因子により形成される細胞増殖に関わる制御ネットワークの解明が腫瘍細胞の制御につながることが期待される。
|
