2021 Fiscal Year Final Research Report

Development of methods to evaluate reactive species' spatial distributions for establishing plasma-bio science bases

Research Project

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Project/Area Number	19H01889
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 14030:Applied plasma science-related
Research Institution	Meijo University
Principal Investigator	ITO MASAFUMI 名城大学, 理工学部, 教授 (10232472)
Co-Investigator(Kenkyū-buntansha)	加藤雅士名城大学, 農学部, 教授 (70242849) 村田富保名城大学, 薬学部, 教授 (80285189)
Project Period (FY)	2019-04-01 – 2022-03-31
Keywords	大気圧プラズマ / 低温プラズマ / プラズマバイオ / 気相中活性種密度測定 / 液中活性種密度分布測定 / ラジカル / 活性酸素種 / 活性窒素種
Outline of Final Research Achievements	It was found that the proliferation promoting effect of fibroblasts showed a strong correlation only with the irradiation amount of nitric oxide radical (NO・), and succeeded in clarifying that NO・ was a direct trigger of cell proliferation. In addition, it was found that when fibroblasts were placed about 1 mm from the gas-liquid interface, growth promotion was obtained by 20% or more, but the growth promotion effect could not be obtained at deeper places. . Furthermore, we have succeeded in constructing a system that can measure the absorbance of the liquid phase at a depth of about 10 mm in increments of 440 μs with a spatial resolution of 13 μm. This measurement system revealed that short-lived active species such as NO・ and HOONO are effective in promoting cell proliferation, rather than long-lived active species such as hydrogen peroxide, nitrite, and nitrate ion.
Free Research Field	プラズマバイオ応用
Academic Significance and Societal Importance of the Research Achievements	本研究成果により、気相中のどの活性種をどれくらいの液中深さに存在する培養細胞に照射すると細胞の増殖促進や死滅の効果が表れるかを定量的に高速に空間分布測定することができるシステムの構築に成功した。これらの定量測定データは今後さらに高速化、広帯域化などの改良を加えることで、OHなどの反応性の高い超短寿命活性種密度分布も測定可能となり、気相だけでなく液相での複雑な反応メカニズムの解明に大きく貢献できると考える。本システムで蓄積される新たな知見は，その安全性が強く求められる医療分野や農業分野の応用には、必要不可欠な重要な知見を得ることができ，プラズマを用いたバイオ分野の発展に大きく貢献できると考える。