2021 Fiscal Year Final Research Report
Mass production of cell spheroid based on a vibration-induced flow
| Project/Area Number |
19H02115
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 20020:Robotics and intelligent system-related
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| Research Institution | Chuo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 宏明 中央大学, 理工学部, 教授 (20372427)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 微細操作 / マイクロロボティクス / マイクロ流体 / 振動誘起流れ / 細胞操作 / 細胞スフェロイド / 音響流れ |
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| Outline of Final Research Achievements |
In this research, we proposed mass production method of sell spheroids based on a vibration-induced flow. Vibration-induced flow is local flow induced around vibrating microstructure. Thus, by patterning micropillars in ring arrangement on a chip and applying vibrations to the chip, vortex flow is induced at the center of the structures. By utilizing this vortex flow, we succeeded in forming cell spheroids. Furthermore, by patterning array of the structure, we succeeded in fabricating a few hundred cell spheroids. Next, we developed shape evaluation system of cell spheroids. We also used vibration-induced flow to evaluate 3D shape of cell spheroids. By rotating cell spheroids and observing with microscope, we can evaluate 3D shape of cell spheroids. We succeeded in developing rotation method of microobjects such as cell spheroids and demonstration of feedback control of orientation of microparticles. We will construct 3D shape information of cell spheroids with this system.
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| Free Research Field |
マイクロナノロボティクス
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では，生体に近い特性を持つ細胞凝集塊を大量生産する技術を開発することに成功した．本研究で提案する技術により，数百個の細胞凝集塊を約１０分で作製することが可能となった．この技術により品質の揃った細胞凝集塊を大量生産することが可能となれば，それを用いて新規薬剤の評価を行うことや，再生医療用の生体組織を作製することが実現され，安全・安心な医療を広く提供することが可能となると考えられる．
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