Development of optical tomographic imaging system for in-vivo nano-vibration measurement of whole 3D structure in biological tissue

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H02151
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 21030:Measurement engineering-related
• Research Institution: Niigata University
• Principal Investigator: Choi Samuel 新潟大学, 自然科学系, 准教授 (60568418)
• Co-Investigator(Kenkyū-buntansha): 村松 正吾 新潟大学, 自然科学系, 教授 (30295472) ; 日比野 浩 大阪大学, 医学系研究科, 教授 (70314317) ; 任 書晃 岐阜大学, 大学院医学系研究科, 教授 (80644905) ; 田中 洋介 東京農工大学, 工学(系)研究科(研究院), 准教授 (20283343)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 光コム / 干渉計測 / 光コヒーレンストモグラフィ / スーパーコンティニウム光

Outline of Final Research Achievements

In this study, we aimed to develop an optical tomography imaging system oriented to nano-vibration measurement of the entire 3D structure at the biological tissue. First, we proposed the principle of a heterodyne coherence microscope that can measure the vibration distribution along with a three-dimensional tomographic scan, and demonstrated its usefulness through validation experiments. Next, we constructed a system using a DD-MZM comb generator and proposed a method to realize ultra high-speed measurement at a scan rate of 20 MHz, which enables transient measurement of the vibration state as well as depth profile. Furthermore, by applying the same light source to a spatial interference microscope, it was shown that 3D measurements with nano-order precision over a wide depth range of several hundred micrometers to millimeters are possible. The possibility of the proposed methodwas demonstrated.

Free Research Field

光エレクトロニクス

Academic Significance and Societal Importance of the Research Achievements

メカノバイオロジー分野において、生体内振動現象を３次元的に捉え解析する手法や装置の開発が必須である。また、生体計測分野のみならず、広い範囲の高速なナノ振動の計測と可視化はMEMSや工業製品検査などの生産現場における品質管理等、多岐に渡って重要な課題である。本研究では、これらの社会的要望に対して、光コムを用いた新たな計測手法を提案し、３次元断層計測と振動計測を同時に可能とする装置の開発を進めた。これらの技術が今後社会インフラや工業生産分野、また生体計測分野へ及ぼす波及効果は大きい。