Elucidation of lipid-protein interaction which enhances the amyloid formation

2022 Fiscal Year Final Research Report

• Project/Area Number: 19H02669
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 32010:Fundamental physical chemistry-related
• Research Institution: Saitama University
• Principal Investigator: Otosu Takuhiro 埼玉大学, 理工学研究科, 准教授 (90564948)
• Co-Investigator(Kenkyū-buntansha): 山口 祥一 埼玉大学, 理工学研究科, 教授 (60250239)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 蛍光相関分光法 / 光退色後蛍光回復法 / 脂質二重層膜

Outline of Final Research Achievements

In this project, we sought to develop new spectroscopic tools to quantitatively elucidate the amyloid formation enhanced by the presence of lipid bilayer. We then develop two fluorescence-based techniques during this project. One is called Fluorescence Lifetime Recovery After Photobleaching (FLRAP) which enables us to simultaneously analyze the molecular diffusion of multiple species having their intrinsic lifetime. Another one is the technique that analyzes the dynamical change of lipids during the period when the peripheral molecules attach on the lipid bilayer.

Free Research Field

生物物理

Academic Significance and Societal Importance of the Research Achievements

本研究期間で開発を行った2つの手法はアミロイド研究において重要とされる蛋白質―脂質間相互作用を定量的に解析する手法を提供するのみならず，膜融合過程など様々な生命現象の定量的解析において有用なツールになると期待される．その点において，本研究機関における成果は十分な学術的意義を有するものであると考える．