2021 Fiscal Year Final Research Report
Multi-color super-resolution imaging of organelle communications using super-photostable fluorescent dyes
| Project/Area Number |
19H02849
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 37030:Chemical biology-related
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| Research Institution | Nagoya University |
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Principal Investigator |
TAKI Masayasu 名古屋大学, 物質科学国際研究センター(WPI), 特任准教授 (70378850)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | オルガネラ染色剤 / 蛍光イメージング / 耐光性 / マルチカラー / ミトコンドリア / 脂肪滴 / 超解像イメージング |
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| Outline of Final Research Achievements |
Super photostable organelle fluorescent labeling agents have been developed for long-term observation of various organelle dynamics. We synthesized a series of fluorescent dyes with large Stokes shifts based on a ladder-type pai-skeleton containing phosphole or thiophene rings, as well as near-infrared fluorescent dyes based on the phosphaxanthene skeleton, which have a small Stokes shift. Each of these fluorescent labeling agents was given organelle selectivity. By taking advantage of their excellent photostability, time-lapse imaging of target organelles was achieved with super-resolution microscopy, which requires extremely intense laser irradiation. The combination of these fluorophores allowed multi-color labeling of multiple organelles, enabling real-time observation of organelle changes in real time.
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| Free Research Field |
ケミカルバイオロジー
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| Academic Significance and Societal Importance of the Research Achievements |
生きた細胞においてオルガネラ間に働く相互作用を解析するためには，超解像技術による可視化が有効であり，これを可能にする超耐光性オルガネラ染色剤の開発が必要不可欠である．本研究で得られた成果は，蛍光色素の分子設計において新たな指針を示したのみならず，オルガネラ間連携を解明するうえで強力な技術になるはずである．したがって，生命科学分野，医学・創薬など様々な学問分野への寄与が期待される研究成果であるといえる．
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