Comprehensive analysis SFBB for S-RNase in self-incompatibility of Japanese pear

2022 Fiscal Year Final Research Report

• Project/Area Number: 19H02946
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 39030:Horticultural science-related
• Research Institution: Kobe University
• Principal Investigator: Yasuda Takeshi (高崎剛志) 神戸大学, 農学研究科, 教授 (30314511)
• Co-Investigator(Kenkyū-buntansha): 井上 加奈子 大阪大学, 超高圧電子顕微鏡センター, 講師 (00647737) ; 藤本 龍 神戸大学, 農学研究科, 准教授 (60620375)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 自家不和合性 / ニホンナシ / セイヨウナシ / S-RNase / SFBB

Outline of Final Research Achievements

In this study, we sequenced the BAC contigs of 1.1 kb-1.5 Mb around S2-, S3- and S4-RNases of Japanese pear to find all the SFBB genes encoded by the three S-haplotypes. Using mRNA-sequences and RT-PCR cloning on the pollen derived from seven S-homozygous trees of Japanese pear and three cultivars of Japanese and European pear, other nine haplotypes were analyzed to identify the complete suite of SFBB genes in each haplotype. The SFBB proteins encoded by a total of 12 S-haplotypes of Pyrus species, were classified into 23 types. The same type of SFBB protein share higher degrees of amino acid sequence identity than those of S-RNase, suggesting that the same type SFBB recognize the same non-self S-RNases. The presence or absence of SFBB alleles in each type could predict the non-S-RNases that nine types of SFBBs target.

Free Research Field

果樹園芸学

Academic Significance and Societal Importance of the Research Achievements

ナシ属は雌しべと雌しべをもつ両性花を着生するが, 自家不和合性を示すため自家花粉では受精・結実しないため, 果実生産には他品種の花粉を人工受粉する必要がある。この雌しべと花粉間の自他認識の仕組みを解明することができれば, 果樹の生産や育種では障害となっている自家不和合性の打破技術の開発や自家和合性品種の育成に繋がる。