The molecular mechanisms underlying bacterial wilt pathogen elimination from host plants with antagonistic rhizobacterial treatment

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H02954
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: Gifu University
• Principal Investigator: Shimizu Masafumi 岐阜大学, 応用生物科学部, 准教授 (60378320)
• Co-Investigator(Kenkyū-buntansha): 山本 義治 岐阜大学, 応用生物科学部, 教授 (50301784) ; 石賀 康博 筑波大学, 生命環境系, 助教 (50730256) ; 別役 重之 龍谷大学, 農学部, 准教授 (80588228)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 青枯病菌排除 / 根圏細菌 / 生物防除

Outline of Final Research Achievements

In tomato seedlings treated with a mixture of Mitsuaria sp. TWR114 and Ralstonia sp. TCR112, expression of genes related to nitrate transportation was characteristically induced. Furthermore, the nitrate concentration in xylem sap of the mixed-treated tomatoes was significantly reduced compared to the control tomatoes. However, since the growth of bacterial wilt pathogen in the xylem sap of the mixed-treated tomatoes was similar with that of the control tomatoes, we could not find a relationship between the decreased nitrate concentration and the bacterial wilt pathogen elimination. On the other hand, genome analysis of TWR114 and TCR112 revealed that both bacterial strains possess genes encoding effectors similar to those of bacterial wilt pathogen and genes for the biosynthesis of various antimicrobial substances.

Free Research Field

植物病理学

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、拮抗微生物による植物病害防除のメカニズムに関する新しい知見を提供するものであり、植物病理学的に重要である。また、本研究では、天然単糖であるアラビノースを植物根に処理すると青枯病が抑制されることを副次的に発見したが、この成果は低環境負荷型の新規病害防除剤の開発に繋がる可能性があり、社会的意義は大きい。