Novel adipokines, NT3 and NT4/5 control msenchymal stem cells

2022 Fiscal Year Final Research Report

• Project/Area Number: 19H03116
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Hokkaido University
• Principal Investigator: Kimura Kazuhiro 北海道大学, 獣医学研究院, 教授 (30192561)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 褐色脂肪 / ベージュ脂肪細胞 / エネルギー消費 / UCP1 / 生活習慣病予防

Outline of Final Research Achievements

Neurotrophic factors (NTs) are also expressed in non-neural tissues, but their roles remain unresolved. When the stromal vascular fraction was fractionated from rat white adipose tissue and examined for the effects of NT, cell numbers did not change. However, the addition of a neutralizing antibody decreased cell number, and this decrease was reversed by an extra dose of cytokine. On the other hand, nerve growth factor NGF had no such effect. A similar response was observed in mice, and NT3 and NT4/5 conditioned knockout mice were generated. We are currently using these mice to determine the cell counts of progenitor adipocytes and myoblasts, and their responsiveness to PDGF, a known progenitor adipocyte growth factor, and insulin, as well as differentiation factor expression.

Free Research Field

基礎獣医学

Academic Significance and Societal Importance of the Research Achievements

本研究では、ニューロトロピンNT3及び、NT 4/5のあらたな役割を解明することを目的とした。その結果二つのニューロトロピンが、前駆脂肪細胞の生存や分化因子への応答性を維持することが明らかとなり、一方インスリン依存性の前駆骨格筋細胞においても様々な影響を与えることが明らかとなった。現在NT3とNT4ノックアウトマウスを作成したので最終的な結論を得るために実験を行なっている。結果は、脂肪細胞及び骨格筋細胞の形成過程にニューロトロピンがどのように作用するか明らかにしたものであり前駆細胞の役割を示すものであり生活習慣病対策を考える上で重要である。