Toward understanding regulatory system of spatial gene expression

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H03424
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 48040:Medical biochemistry-related
• Research Institution: Kyoto University
• Principal Investigator: Oki Shinya 京都大学, 医学研究科, 特定准教授 (90452713)
• Co-Investigator(Kenkyū-buntansha): 大川 恭行 九州大学, 生体防御医学研究所, 教授 (80448430)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 空間オミクス

Outline of Final Research Achievements

For tissue-specific gene expression and epigenomics analysis, specific cell populations from individuals and organs must be "isolated" with high purity. However, laser microdissection is difficult to isolate small cell populations, and cell sorting inevitably causes damage when cells are suspended by enzymatic treatment. In this study, we developed a completely new method, Photo-Isolation Chemistry, which is different from such "sorting," and succeeded in transcriptome analysis limited to the photo-irradiaterd area.

Free Research Field

空間オミクス

Academic Significance and Societal Importance of the Research Achievements

ROIに対するUV照射の分解能は、光学限界のサブミクロンオーダーまで高められる。したがって１細胞レベルのROIに照射できるうえ、特定のオルガネラに局在する転写産物も分離できる。本手法は、caged オリゴDNAさえあればだれでも実験できるため、急速に普及することが期待される。また、本研究提案の根幹技術はATAC-seqやChIL-seqにも適用できると考えられるため、将来、組織特異的なエピゲノミクスデータを取得できると期待される。