2021 Fiscal Year Final Research Report
Regulatory mechanism of transcription by ciliary proteins.
| Project/Area Number |
19H03447
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 49030:Experimental pathology-related
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| Research Institution | Kagawa University (2021)
Hokkaido University (2019-2020) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
徳光 浩 岡山大学, ヘルスシステム統合科学学域, 教授 (20237077)
平田 徳幸 北海道大学, 遺伝子病制御研究所, その他 (40595956)
松岡 達臣 高知大学, 教育研究部自然科学系理工学部門, 教授 (90209510)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 一次繊毛 / 転写 / シスト / 細胞極性 |
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| Outline of Final Research Achievements |
Inversin accumulates in a Cajal body-like structure in the nucleus, which is one of the centers of transcriptional activity, and has a specific transcriptional regulatory activity. This strongly suggests that the ciliary protein Inversin functions as a novel transcriptional regulator. In addition, it was clarified that during the induction of cyst formation in the unicellular ciliate Colpoda cucullus, rapid fragmentation and expression change of the ciliate constituent protein beta-tubulin occurred and it was taken up into the body. From the dynamic changes of ciliary proteins, it is considered that the localization, structure, and functional changes of ciliary proteins and reabsorption lead to the reuse of amino acids and nucleic acids for cyst formation. In addition, it is presumed that changes in the transcriptional control function of ciliary proteins are important because they acquire the properties of low temperature, low pH, and UV resistance with the rapid change to cysts.
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| Free Research Field |
細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
細胞性一次繊毛は、外界の環境変化を素早く感知し生物反応するための重要な細胞器官である。そのため様々な増殖因子受容体やイオンチャネルなどの重要なタンパク複合体が繊毛特異的に蓄積され、繊毛は細胞内へのシグナル伝達のハブとして機能する。本研究の目的である繊毛タンパクが転写因子として直接機能することを明らかにすることで、細胞の迅速な生物反応と恒常性、増殖、分化制御における繊毛の生物機能の分子メカニズムを解明出来ると考える。本研究成果により、一次繊毛機能の破綻が惹起する繊毛病やがんなどの疾患新規診断・治療法開発へつながり、基礎生物学から臨床研究への波及効果が期待できる。
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