Significance of glycan abnormalities in diabetic nephropathy

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H03675
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 53040:Nephrology-related
• Research Institution: Okayama University
• Principal Investigator: Wada Jun 岡山大学, 医歯薬学域, 教授 (30294408)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 糖尿病性腎症 / 尿レクチンアレイ / 網羅的糖鎖プロファイル / Ｃ型レクチン受容体

Outline of Final Research Achievements

Urinary lectin array analysis was successfully developed by using 45 lectins with different glycan binding specificity. In the cohort of patients with type 2 diabetes (n=680), higher glycan binding signals to SNA, ABA, Jacalin, ACA, and RCA120 predicted the 30% eGFR decline after 4 years follow-up. The lectin histochemistry demonstrated that T-antigen, Tn-antigen, sialyl-T, sialyl-Tn, and Core 3 recognized by these lectins were localized proximal tubular cells and secreted to the urine.

Free Research Field

腎臓病学、糖尿病学

Academic Significance and Societal Importance of the Research Achievements

糖尿病性腎症において近位尿細管細胞に発現するTn-antigenやsialyl-Tnと、マクロファージ上に存在しそれらの糖鎖構造を認識するC型レクチン受容体の相互作用が進展に関与している可能性を示した。今後の糖尿病性腎症の診断や治療の開発への研究の展開が期待される。