Dental implant treatment and mesenchymal stem cell aging

2022 Fiscal Year Final Research Report

• Project/Area Number: 19H03843
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 57050:Prosthodontics-related
• Research Institution: Kyushu University
• Principal Investigator: AYUKAWA Yasunori 九州大学, 歯学研究院, 教授 (50304697)
• Co-Investigator(Kenkyū-buntansha): 熱田 生 九州大学, 歯学研究院, 准教授 (30423487) ; 古谷野 潔 九州大学, 歯学研究院, 特別教員 (50195872)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 間葉系幹細胞 / Stem cell aging / 歯科インプラント

Outline of Final Research Achievements

The purpose of this study was to investigate aging and disease MSCs and bone dynamics in peri-implant bone formation. First, rats with osteonecrosis of the jaw were generated, and MSCs were compared with healthy MSCs. Some protein and gene expression was inferior in the latter, and the expression of some proteins that were decreased in the latter tended to be restored by co-culturing with healthy mouse MSCs. In addition, osteonecrosis did not develop in the surrounding bone of implants placed in a tibia model of osteonecrosis of the jaw, suggesting that the oral environment affects the development of osteonecrosis.
Next, we examined differences in the cross-linking structure of bone collagen, and found that type I collagen fibers from mice with osteonecrosis of the jaw tended to be degraded.

Free Research Field

歯科補綴学

Academic Significance and Societal Importance of the Research Achievements

本研究計画では、「加齢関連口腔領域疾患には間葉系細胞（MSC）の老化（stem cell aging: SCA）が影響している」、「SCAを制御することが加齢関連口腔領域疾患の制御につながる」という仮説を検証することを目的として企画した。研究の結果、MSCの疾患による劣化、健全MSCによる劣化MSCの健全化が再確認された。また、顎骨壊死（MRONJ）は脛骨では発症しなかったことから、MRONJ発症における口腔環境の重要性が示唆された。さらに、MRONJはコラーゲンの架橋構造を変化させることが示唆され、MRONJの治療戦略に貢献しうるデータが提供できた。