Elucidation of mechanism on congenital abnormality in the maxillofacial region focusing on DNA repair mechanism

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H03849
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Niigata University
• Principal Investigator: MAEDA TAKEYASU 新潟大学, 医歯学系, 教授 (40183941)
• Co-Investigator(Kenkyū-buntansha): 佐伯 万騎男 新潟大学, 医歯学系, 教授 (30273692) ; 大峡 淳 新潟大学, 医歯学系, 教授 (40266169) ; 柿原 嘉人 新潟大学, 医歯学系, 助教 (40379938)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: DNA修復機構 / Reptin / 先天異常 / 酸化ストレス

Outline of Final Research Achievements

DNA repair is essential process for cell survive. It remains unclear whether DNA repair system is required for development. We generated mice with epithelial conditional deletions of DNA repair-related molecule, Reptin. Reptin cKO showed the arrest of epidermis development. The anomalies of epidermis were found to be caused by DNA damages and subsequent inhibition of cell proliferation due to aberrant activation of p53/p21 signals. We found that oxidative stress damage DNA during normal skin development. DNA damage caused by oxidative stress is repaired by Reptin, which lead to normal skin development.

Free Research Field

口腔組織・発生学

Academic Significance and Societal Importance of the Research Achievements

DNAは紫外線や化学物質などの影響で日々損傷を受けているが、DNA修復分子機構により修復されることで、正常な機能が維持される。この修復機構が破綻すると癌などの疾患を引きおこす。胎生期にもDNAの修復機構が必要であることを示した本研究結果は、先天異常の原因の理解や、先天異常の生前治療に新たな展開をもたらす可能性を持つ。また、DNA修復機構の基盤的知見ともなる