Analysis of carcinogenesis mechanism using PDX model based on exome analysis in Japanese oral cancer

2023 Fiscal Year Final Research Report

• Project/Area Number: 19H03855
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Tokai University
• Principal Investigator: Ota Yoshihide 東海大学, 医学部, 教授 (60233152)
• Co-Investigator(Kenkyū-buntansha): 木村 穣 東海大学, 医学部, 客員教授 (10146706) ; 田嶋 敦 金沢大学, 医学系, 教授 (10396864)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: 口腔扁平上皮癌 / OSCC / PDXモデル / 次世代シーケンス / エクソーム解析

Outline of Final Research Achievements

This study aimed that analysis of oral squamous cell carcinoma using the Patient-derived xenograft (PDX) models which was established by us. 3 of 17 primary transplants were successful. The second transplantation was successful in 2 out of 3 cases, and the third transplantation was successful in 2 out of 2 cases. We analyzed mutation analysis in tumor of PDX models and patients which was donor. We detected 73 mutations and 371651 mutations in PDX models and donor patients. We were able to establish PDX model mouse derived from OSCC. Furthermore, the tumor of PDX models reflected the genetical features in donor patients.

Free Research Field

口腔がん

Academic Significance and Societal Importance of the Research Achievements

現在までに、頭頸部扁平上皮癌のPDXモデルはカリフォルニア大学サンフランシスコ校のグループ(Li ら)が発表しているが、OSCCサンプルは含まれておらず、肺がんのPDXモデルの分譲をしているジャクソン研究所を含め、他には発表がない。そのため、本研究の成果はOSCCの病態解明を発展させる可能性が高いと考える。さらに、PDXモデルは薬剤開発の前臨床試験にも用いるため、本研究のモデルマウスは新規治療戦略の開発にもつながると考える。