Amplified luminescence proximity homogeneous assay for asbestos contamination

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H04291
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 64010:Environmental load and risk assessment-related
• Research Institution: Hiroshima University
• Principal Investigator: Kuroda Akio 広島大学, 統合生命科学研究科(先), 教授 (50205241)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: アスベスト / アスベスト結合タンパク質 / バイオアッセイ / 化学発光 / AlphaLISA

Outline of Final Research Achievements

A number of methods have been developed to assess asbestos contamination in construction materials using microscopy and X-ray diffraction. However, adaptation of these methods to high-throughput analysis has been difficult. Amplified luminescence proximity homogeneous assay (Alpha) technology relies on binding of two different beads to a target of interest. In case of the binding and the resulting proximity of the two beads, an energy transfer from one bead to the other takes place. This results in the production of a chemiluminescent signal. Here we developed two different beads that display asbestos binding proteins and demonstrated that this technology would be applicable to high-throughput analysis of asbestos.

Free Research Field

生物工学

Academic Significance and Societal Importance of the Research Achievements

アスベストは、建材に広く使用された繊維状ケイ酸塩鉱物である。アスベスト繊維の吸入は肺に損傷を与え、胸膜中皮腫などの深刻な健康問題を引き起こすことが知られている。アスベスト建材を含む解体工事は年間約188万棟あるので、分析すべき建材数が膨大な数にのぼる。アスベストの迅速なハイスループット検出技術は国民の安全安心に資する。