2023 Fiscal Year Final Research Report

Development of plasma bubble spray administration and clinical application to gastrointestinal cancer

Research Project

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Project/Area Number	19K03814
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 14030:Applied plasma science-related
Research Institution	Tokyo City University
Principal Investigator	MORI AKIRA 東京都市大学, 理工学部, 教授 (60219996)
Co-Investigator(Kenkyū-buntansha)	小林千尋東京都市大学, 理工学部, 講師 (00570699) 工藤美樹広島大学, 医系科学研究科(医), 教授 (80241082) 平田孝道東京都市大学, 理工学部, 教授 (80260420)
Project Period (FY)	2019-04-01 – 2024-03-31
Keywords	がん治療 / 大気圧低温プラズマ / プラズマ照射水
Outline of Final Research Achievements	Colorectal cancer affects a large number of people. In recent years, research on medical applications of atmospheric low temperature plasma (ALTP) has been actively conducted. In vivo and in vitro experiments were conducted using plasma-treated saline (PTS) and plasma-treated medium (PTM) prepared by submerged bubbling treatment using ALTP. In vivo experiments using a rat model of colorectal cancer revealed that PTS administration to the colon slowed tumor progression based on endoscopic and histopathological observations. As a result of culturing colorectal cancer cells with PTM, cell proliferation was inhibited. Also, cell death was induced by cell swelling. The qPCR revealed that PTMs induced cell death of cancer cells through signaling of tumor necrosis factorα(TNF-α), an inflammatory cytokine. Therefore, the inhibition of cancer progression by PTS in the rat model occurs by inducing cell death through the dissolution of ALTP components in the liquid.
Free Research Field	プラズマ医学
Academic Significance and Societal Importance of the Research Achievements	本研究ではin vivo、in vitroの両方の側面からプラズマ照射溶液の評価を行った。大腸癌細胞単体への評価として、培養液プラズマ照射群の細胞数が有意に減少した。更に、大腸癌モデルラットでの評価で病理組織検査の結果から、プラズマ照射生理食塩水液を尾静脈注射した群では臓器転移や深部浸潤抑制の傾向が見られた。従って、本研究によりプラズマ照射溶液が大腸癌細胞死と転移抑制の作用を有することが示唆された。今後は、プラズマ照射溶液による大腸癌細胞抑制作用機序を検討することが必要である。癌治療において手術、放射線、薬物療法でも効果が認められない場合にプラズマ医療も考慮される可能性がある。