2021 Fiscal Year Final Research Report
Effects of stoppage evasion of active transport and pressurized dissolution of a hydrophobic gas on cellular damage in cold storage.
| Project/Area Number |
19K04222
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 19020:Thermal engineering-related
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 冷温保存 / キセノンガス加圧溶解 / 能動輸送停止の回避 / 細胞保護効果 / 電気インピーダンス計測 |
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| Outline of Final Research Achievements |
As a result of this research task, the electrical impedance measuring method for discovering the boundary temperatures of the active transport stoppage under cold storage was established first. And it was suggested to the active transport of the rat heart striated myocyte in cold storage that a stoppage is avoided at 6 degrees C.
Next, the existence of an active transport stoppage of the cell in cold storage, relevance with a cell damage, and the availability of pressurized dissolution of xenon gas at a temperature higher than a boundary temperature were clarified. Namely when pressurized dissolution of xenon gas was performed, specifically, protective effect of cells showed high value at a storage time till 24 hours in the range of 4-6 degrees C especially. Moreover, the active transport stoppage became a factor which reduces the rate of cell viability greatly, and the decrease of the insulation resistance by loss of the completeness of a cell membrane was suggested as the result.
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| Free Research Field |
生体熱工学
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| Academic Significance and Societal Importance of the Research Achievements |
次の3つの意義を合わせて移植医療や再生医療に貢献できる．
1）冷温保存で用いる能動輸送停止温度（0～4℃）に対しそれを回避した温度（6℃）の保存への有効性が示唆された．保存可能時間の延長に繋がる可能性がある．2）キセノンガス加圧溶解は冷温保存した細胞に高い保護効果を示すことから，保護物質に用いることで保存可能時間の延長に繋がる．また，保存中のみ効果を働かすことが出来，保存後の減圧で自然除去となることが画期的である．3）電気インピーダンス計測による能動輸送停止の判別の機序として細胞膜のダメージによる電気的性質の変化が示唆された．これは細胞生存率にも直結するため，生存率評価への利用にも繋がる．
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