2021 Fiscal Year Final Research Report
Development of high-speed cell sheet stacking method to achieve high transplantation efficiency
| Project/Area Number |
19K04281
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 20010:Mechanics and mechatronics-related
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 再生医療 / 組織工学 / 細胞シート / 心筋細胞 / 心不全治療 / 立体組織 / フィブリン / iPS細胞 |
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| Outline of Final Research Achievements |
In this study, we have developed a method for constructing a three-dimensional tissue by multi-layering cell sheets at high speed and transplanting a large amount of cells into a living body. Recently, cell sheets have reached clinical application for diseases such as cornea and heart. However, transplantation has a limit in the amount and thickness of tissue that can be supplemented by diffusion of oxygen and nutrient supply. Therefore, we designed an effective cell transplantation therapy by creating transplanted tissue at high speed and connecting the vascular network in the reconstructed tissue with the blood vessels on the host side. As a result, we established a method to significantly improve cell transplantation efficiency by sandwiching fibrin gel that promotes angiogenesis between each cell sheet when laminating and constructing 10-la
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| Free Research Field |
生体医工学・組織工学・再生医療
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| Academic Significance and Societal Importance of the Research Achievements |
細胞シートを大量に移植が可能となり長期的に生着すること確立できれば、将来的に様々な応用が考えられる。従来では数層の上皮および、間葉系細胞シートしか実現していない。本研究成果では10層もの心筋細胞シートが移植が可能になった。これは、欠損した組織を補完する治療ができる可能性を示しており、心筋梗塞または拡張型心筋症によって欠損した心筋細胞を本技術によって補完できる。したがって、本提案技術は未だに確立していない欠損部位に対する組織移植治療が実現できる技術になり、次世代細胞・組織治療法になる。
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