Single-cell metabolomic and proteomic analyses in cancer cells by a novel mass spectrometry-based analytical system

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05167
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 27040:Biofunction and bioprocess engineering-related
• Research Institution: Kyushu University
• Principal Investigator: Izumi Yoshihiro 九州大学, 生体防御医学研究所, 准教授 (70622166)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 1細胞メタボローム解析 / 1細胞プロテオーム解析 / シングルセル / 1細胞解析 / がん細胞

Outline of Final Research Achievements

Heterogeneous cell population including rare cancer cells (e.g., circulating tumor and stem cells) in blood and tissues is related to cancer development and drug resistance. Thus, information garnered from the analysis of samples extracted from a rare cancer single-cell is expected to advance knowledge of the mechanism of cancer development and the discovery of therapeutic strategy. However, mass spectrometry-based conventional proteomic and metabolomic analyses require large amount of starting materials (e.g., 10^5-10^6 cells) due to technical limitations. The aim of this study is to develop a novel analytical system for single-cell proteomics and metabolomics in mammalian cells by a combination of single-cell sampling, in-line sample preparation techniques, and highly sensitive nanoflow liquid chromatography tandem mass spectrometry (nano-LC/MS/MS).

Free Research Field

オミクス解析

Academic Significance and Societal Importance of the Research Achievements

本研究で開発した1細胞分子フェノタイプ解析システムは，申請者らの独自の要素技術が集約されたものであり，技術レベルはきわめて高い．今後，各種疾患関連試料の1細胞分子フェノタイプ解析を推進していくことで，従来の平均的観測では見出すことのできなかった重要なタンパク質や代謝物を発見し，それらの知見を基に疾患の機序解明あるいは鋭敏なバイオマーカー候補の探索に応用できる可能性がある．将来的には，当該技術はライフサイエンスの幅広い分野において貢献できると考えられる．