2021 Fiscal Year Final Research Report
Development of on-site rotary rod device for collection and removal of biogenic ions from surface of cells and tissue
Project/Area Number |
19K05523
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 34020:Analytical chemistry-related
|
Research Institution | Toyohashi University of Technology |
Principal Investigator |
Hattori Toshiaki 豊橋技術科学大学, 工学(系)研究科(研究院), 准教授 (80198762)
|
Project Period (FY) |
2019-04-01 – 2022-03-31
|
Keywords | 生きた細胞実験 / 薬剤の回収・除去 / 繰り返しのできる生物実験 / キレート樹脂の吸収特性 / 分解酵素の固定技術 |
Outline of Final Research Achievements |
Experiments using living cells are generally considered hard to repeat. In this study, we developed a method to collect and detoxify stimulus compounds simultaneously with local its drug release as a way to realize live cell studies that can be repeated experiments. As for the metal ion recovery method, actual measurements and simulation results show that chelating resins can significantly reduce calcium ion concentrations by mechanism other than diffusion. This was a very interesting and new discovery. In addition, by developing a method that allowed the degradative enzyme to be firmly fixed to the rotating rod, we succeeded in converting the stimulus compound to a non-stimulant compound and immediately lowering the concentration of the stimulus compound.
|
Free Research Field |
分析化学
|
Academic Significance and Societal Importance of the Research Achievements |
生きた細胞を用いた実験は、細胞の準備に専門的な技術と様々な注意が必要です。また、その準備にはかなりの時間と多くの労力が必要です。そのため、何度も繰り返して再現性のあるデータ(情報)を得るにはかなりの日数と手間がかかってしまいます。そこで、本研究では、繰り返しが可能な生きた細胞実験を実現する手法としての刺激薬物の回収・除去技術を開発しました。この手法を発展させると、様々な生きた細胞に対する薬物実験が何度も繰り返してデータを得ることが容易になります。すなわち、本研究は生物の代謝機能を明らかにする実験、および、新たな創薬の開発のための実験を促進するのに役立つ研究です。
|