Utilization of "antibodies" using "paper" as a medium, which is versatile and ultra-sensitive

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05553
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 34020:Analytical chemistry-related
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Hideto Hoshino 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (20371073)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: VHH抗体 / セルロース / プロテアーゼ / RNase / 蛍光蛋白質 / 紙 / セルロースアダプター

Outline of Final Research Achievements

We applied our original technology, "-C-Link" to VHH antibody, and tried to develop and advance the general-purpose platform technology "VHH-C-Link" of VHH antibody that can utilize VHH antibody more freely.
Specifically, we had two approaches, one was the development of a protease-sensor integrated lateral flow device, in which an anti-GFP VHH antibody was placed on the test line using the activity of a protease that cleaves a peptide bond with a specific amino acid sequence as an index, and the other the development of a new method of utilizing anti- bovine RNaseA VHH antibody so that captures the human ortholog hRNase1 and uses its RNA degradation activity as an index. Through these studies, we have presented the new possibility of utilizing VHH antibodies that do not follow the conventional IgG utilization method.

Free Research Field

蛋白質工学

Academic Significance and Societal Importance of the Research Achievements

VHH抗体は蛋白質工学的な改良が容易な点に利点があるにも関わらず、その優位性を従来技術では発揮できていない。当該研究では、極めてシンプルな原理に基づく、低コストで環境負荷が低い検査手法を検討した。それぞれの手法には更なる高度化の余地が残されるが、従来の抗体活用技術とは明確に一線を画すVHH抗体の新しい活用プラットフォーム基盤の提案には、学術的意義のみならず、大腸菌生産蛋白質とセルロース素材という安価で天然の素材の複合化による低コスト検査の可能性を示した点に、医療費を抑える必要性に迫られている、或いは環境負荷を意識せざるを得ない現代社会に対する社会的意義がある。