Analysis of signaling network controlling the expression of carbohydrate-degrading enzyme genes in filamentous fungi

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05777
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Osaka Prefecture University
• Principal Investigator: Tani Shuji 大阪府立大学, 生命環境科学研究科, 准教授 (80405357)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 遺伝子発現制御 / 糸状菌 / 酵素生産

Outline of Final Research Achievements

The aim of this study was to elucidate the regulation mechanism of carbohydrate-degrading enzyme genes in Aspergillus aculeatus, especially to analyze the mode of action of four novel factors, ClbR, Uge5, DppIV, and SrpkF. The research was conducted in the following areas. We have restricted the domain in ClbR involved in the transcription activation. We revealed that Uge5, which has UDP-glucose 4-epimerase activity, is involved in the regulation of gene expression of mannanase as well as cellulolytic enzymes, that the dipeptidyl activity of DppIV is involved in the regulation of cellulase gene expression, and that the cellulase gene expression is regulated by the dipeptidyl activity of DppIV. We also found that modification of the putative phosphorylation domain of SrpkF enhanced the enzyme expression level in A. aculeates.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

植物細胞壁を構成する多糖を酵素により分解し、得られた糖から有用物質を生産する技術は、持続可能な社会基盤を構築することに資する技術として注目されている。この技術革新に向けて改善すべき課題の一つに酵素生産コストの削減があげられる。本研究でたんぱく質リン酸化酵素の機能を改変することで、酵素生産を誘導するための物質がない条件化でも酵素生産を促進することを可能にした。本研究成果は、酵素生産コスト削減に資するものである。