2022 Fiscal Year Final Research Report
Explotiation of probiotic system capable of reducing peroxides
Project/Area Number |
19K05780
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 38020:Applied microbiology-related
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Research Institution | Tokyo University of Agriculture |
Principal Investigator |
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Project Period (FY) |
2019-04-01 – 2023-03-31
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Keywords | 過酸化脂肪酸還元乳酸菌 / プロバイオティックス / 過酸化脂肪酸 / 過酸化物還元 / 抗酸化ストレス |
Outline of Final Research Achievements |
Reinforcement of hydroperoxide-eliminating activity in the intestines and colon should prevent associated diseases. We successfully isolated an alternative lactic acid bacterium, Lactobacillus plantarum P1-2, that can efficiently reduce environmental fatty acid hydroperoxides to hydroxy derivatives through a 2-electron reduction. The strain exhibited a wide concentration range with regard to the environmental reducing activity for hydroperoxide. Given this, the isolate orally administered to the oxygen-sensitive short-lived nematode mutant, and this resulted in a significant expansion of its lifespan. This observation suggests that L. plantarum P1-2 inhibit internal oxidative stress. To determine the specific organs involved in this response, we performed a similar experiment in rats, involving induced lipid peroxidation by iron-overloading. We observed that only L. plantarum P1-2 inhibited colonic mucosa lipid peroxidation in rats with induced oxidative stress.
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Free Research Field |
応用微生物学
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Academic Significance and Societal Importance of the Research Achievements |
腸には疾病防御の要であり、過酸化物は各種疾病の原因物質であるが、腸の過酸化物分解活性は通常組織より低い。従って、積極的に菌体外過酸化脂肪酸を還元できる乳酸菌が分離できれば、その生菌剤による腸内疾患の防止や治療の可能性が期待されるが、研究報告は見いだせない。 私達は、目的の乳酸菌、Lactobacillus plantarum P1-2株の分離に成功し、線虫の酸素感受性短寿命突然変異体投与では、酸化ストレス防御効果が認められた。そこで、ラットに供試したところ、乳酸菌投与群に大腸粘膜中の脂質過酸化指標値の低下を認めた。乳酸菌による生体内過酸化脂肪酸還元は学術的、応用面での発展が期待される。
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