Development of acid inducible promoter in Eshcherichia coli

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05797
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Ishikawa Prefectural University
• Principal Investigator: Nakagawa Akira 石川県立大学, 生物資源環境学部, 講師 (10573107)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 大腸菌 / 微生物発酵 / 合成生物学 / プロモーター工学

Outline of Final Research Achievements

The purpose of this study is to construct an acid induction system for the developing an inexpensive gene induction promoter. According to analysis of known acid-induced promoters, we successfully constructed an acid-inducible 200 bp promoter by modifying the gadE promoter. However, the expression level of this promoter was low. Next, when the basal transcription region was strengthened, the expression level increased, but the acid inducibility weakened. Therefore, when the strengthened basic transcriptional control region was slightly weakened, acid inducibility was found again. Furthermore, protein overexpression was performed using this promoter, and although acid-induced activity was found in the cells, no clear protein overexpression was achieved.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

化成品は基本的に化学合成法で生産されるが、植物の有効成分等複雑な分子は化学合成法では難しいことがある．その場合、例えばアミノ酸等は、微生物の発酵技術で生産される事がある．微生物発酵生産では反応を促進するタンパク質を過剰に作らせる必要があるが、過剰に作らせるには現状ではコストがかかってしまう．本研究は安価な酸を加えるだけでタンパク質を過剰に作らせることができる可能性を秘めており、安価な化成品生産に貢献できると考えられる．