Activation of sleeping or weak expressing microbial enzyme genes and elucidation of the awakening mechanism

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05811
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38030:Applied biochemistry-related
• Research Institution: University of Yamanashi
• Principal Investigator: Funane Kazumi 山梨大学, 大学院総合研究部, 教授 (90353953)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: リボゾーム工学 / 環状イソマルトオリゴ糖合成酵素 / キシラナーゼ

Outline of Final Research Achievements

Paenibacillus agaridevorans T-3040 is a cyclic isomaltooligosaccharide-producing strain. From the genomic information of this strain, it was estimated that it has two types of xylanase genes. They are predicted to be xylobiose and xylose-producing enzymes, respectively. Two mutants with increased xylanase activity were obtained by the ribosome engineering method. It was confirmed by real-time PCR that the expression levels of the two xylanase genes in the mutant strains were higher than those in the wild strain. On the other hand, bacterial strains which produce xylanase, ιcaraginase, agarase, and glucansucrase were isolated. Polyethylene terephthalate-degrading bacteria and polystyrene-degrading bacteria were also isolated from soil. They were bred by the ribosome engineering method.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

本研究は、放線菌の抗生物質生産目的に研究開発されてきたリボゾーム工学法（リボゾーム関連遺伝子の自然突然変異導入法）が、休眠している酵素遺伝子も覚醒させるのではないかと考え、複数の「休眠酵素遺伝子」の覚醒およびそれらの休眠・覚醒メカニズムの解明を目指した。リボゾーム工学的育種により環状イソマルトオリゴ糖合成酵素と2種類のキシラナーゼの生産力が同時に高まった変異株を得ることができ、この方法を工業用酵素生産微生物にも適用できる可能性を示した。