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2021 Fiscal Year Final Research Report

Development of inhibitor evaluation system for channel proteins based on cell-free system

Research Project

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Project/Area Number 19K05815
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38030:Applied biochemistry-related
Research InstitutionEhime University

Principal Investigator

Nozawa Akira  愛媛大学, プロテオサイエンスセンター, 准教授 (30432800)

Project Period (FY) 2019-04-01 – 2022-03-31
Keywordsチャネル / 無細胞タンパク質合成法
Outline of Final Research Achievements

A complete in vitro channel analysis system has been successfully constructed, in which channel proteins are synthesised in a cell-free system and their activity is detected by a lipid planar bilayer method. Using the human sodium channel Nav1.7 and the chloride channel GABAAR as model proteins, a cell-free synthesis method was investigated and the activity was successfully detected using Nanion Technology's Orbit mini. This system was also successfully used to confirm the effects of inhibitors.

Free Research Field

タンパク質科学

Academic Significance and Societal Importance of the Research Achievements

人工的にタンパク質を合成する合成法と、人工脂質平面膜上にタンパク質を再構成し活性を測定する方法を組み合わせることで、膜輸送体タンパク質の一つであるチャネルタンパク質の基質輸送活性を、完全に人工的な実験系で構築することに成功した。この系は単一のタンパク質の活性を解析できることから、生体内では困難な単一タンパク質の活性評価が可能になる。また、この系で阻害剤の効果も検出できたことから、阻害剤の評価系としても利用可能である。

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Published: 2023-01-30  

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