2022 Fiscal Year Final Research Report
Functional analysis of the peroxisomal transporter ABCD1 in Solanaceae plants
Project/Area Number |
19K05820
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 38030:Applied biochemistry-related
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Research Institution | Kobe Pharmaceutical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
肥塚 崇男 山口大学, 大学院創成科学研究科, 准教授 (30565106)
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Project Period (FY) |
2019-04-01 – 2023-03-31
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Keywords | タバコ / ペチュニア / ABCD1 / ペルオキシソーム / 輸送体 |
Outline of Final Research Achievements |
Most plant metabolites are biosynthesized via several organelles. Peroxisomes are involved in the biosynthesis of secondary metabolites. The involvement of ABCD1 transporters in the intermediates transport has been suggested, but little is known about the transport function and environmental responses. In this study, we examined ABCD1 using petunia and tobacco as models. In cultured tobacco cells, ABCD1 gene expression was induced by yeast extract and jasmonic acid treatment. In tobacco plants, a constant expression level was observed in all tissues. In petunia plants, the ABCD1 gene was also expressed in all tissues, especially in leaves and petals. These findings provide new insights into the mechanism of metabolites biosynthesis in Solanaceae plants.
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Free Research Field |
植物分子生物学
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Academic Significance and Societal Importance of the Research Achievements |
代謝産物は、化合物の同定や生合成経路に関する多くの先行研究があるが、代謝産物の細胞内での空間制御機構の知見は少ない。本研究では、タバコとペチュニアというアルカロイドやベンゼノイド(香気成分)を生産するナス科植物でのペルオキシソーム輸送体を解析した。ABCD1が香気成分生産部位で比較的に発現が高いことなどを明らかにしており、植物の生産する有用産物の生合成機構の理解に新たな知見を提供した。今後、ABCD1をさらに解明、また活用していくことで、植物の環境適応の理解が進むとともに、より香気成分生産を促進させた植物の分飼育種などにつながると期待される。
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