2021 Fiscal Year Final Research Report
Development of convergent synthesis of LPS Internal core oligosaccharides and recognition sites of bactericidal human antibodies
| Project/Area Number |
19K05845
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38040:Bioorganic chemistry-related
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | リポ多糖 / コア糖鎖 / ワクチン / 立体選択的合成 / 糖鎖ライブラリー / 糖鎖合成 |
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| Outline of Final Research Achievements |
We have established a stereoselective synthesis of inner-core oligosaccharide (core OS) of lipooligosaccharide produced by Neisseria meningitidis and N. gonorrhoeae. Using this methodology, we prepared a library of partial core OS of Neisserial LOS and Haemophilus LPS. Furthermore, we prepared conjugates of synthetic glycans with magnetic particles and lipids and established a synthetic method for bio probes. Using magnetic particles modified with synthetic glycans, we found the presence of glycosylated human antibodies in commercially available human antibodies IgG and IgM.
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| Free Research Field |
糖鎖工学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では，髄膜炎菌や淋菌が産生する複合糖質の糖鎖を単一構造として得る化学合成方法を確立した．さらに合成した糖鎖を認識するヒト抗体の存在を確認した．この成果は髄膜炎や淋病に対する感染予防ワクチン開発に貢献することが可能であるだけでなく，他の病原性グラム陰性菌の感染予防ワクチン開発にも応用が可能である．また合成した糖脂質は，自然免疫における糖鎖の機能解明に貢献が可能である．
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