2021 Fiscal Year Final Research Report
Biosynthetic and ecological analysis of organohalogen compounds produced in the cellular slime mold fruiting bodies.
Project/Area Number |
19K05850
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 38040:Bioorganic chemistry-related
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Research Institution | Sophia University |
Principal Investigator |
Saito Tamao 上智大学, 理工学部, 教授 (30281843)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | 有機ハロゲン化合物 / 生合成 / ポリケタイド合成酵素 |
Outline of Final Research Achievements |
Dictyostelium has novel polyketide synthase (PKS), that is a fusion of type-I and type-III PKSs. SteelyB is one of these fusion type PKSs that are conserved across slime mould species but have not been reported from other organisms. SteelyB makes differentiation signaling molecule in early developmental stage. In the fruiting body stage, that is the final stage of development, SteelyB changes its products and makes Chlorinated dibenzofurans including LCC-1. To do this, SteelyB switches its expression from prespore to stalk cells and is cleaved off the type-III PKS domain. This change of expression locus seemed to be the key for the products change. LCC-1 showed strong antibacterial activity, and this suggested that it may protect the fruiting body from bacterial infection. In the soil condition, stlB null strain made tiny fruiting bodies with small spore head. This indicates that Chlorinated dibenzofurans are ecological compounds and are also involved in the fruiting body formation.
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Free Research Field |
生物有機化学
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Academic Significance and Societal Importance of the Research Achievements |
本研究では細胞性粘菌にのみ見られるSteely酵素を構成するI型とIII型PKSの融合と分離の意義を考えた。融合はIII型PKSの基質特異性の低さを補い単一の産物の合成に寄与し、分離は特異性の低さと酵素活性の高さを利用して複数の産物を合成することにつながることがわかった。この成果は生体内でのIII型PKSの機能制御の仕組みを理解するもので、生物を利用した有用物質創出に波及効果を持つ。また、本研究は有機塩化化合物の生態学的な意義の理解も目指したもので、その結果はAmpicillinと同等の最小生育阻止濃度を持つ抗菌物質を見つけ出すことにつながった。
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