2022 Fiscal Year Final Research Report
Development of the DNA markers associated with the agronomically important traits in rose using a tetraploid population
| Project/Area Number |
19K06016
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 39030:Horticultural science-related
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| Research Institution | Meijo University (2022)
Nagoya University (2019-2021) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
落合 正樹 岐阜大学, 応用生物科学部, 助教 (80755827)
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | バラ / トゲ / 花弁形状 / 次世代シーケンサー / GRAS-Di / アソシエーション解析 |
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| Outline of Final Research Achievements |
In this research, we performed the genome-wide association analysis (GWAS) of the prickle density. We also investigated whether we can perform the GWAS of the petal shape in rose by using a tetraploid population. For the former, the GWAS showed a peak on chromosome three, and single nucleotide polymorphisms in this region tend to be biased to the prickle-formed 'PEKcougel' allele. For the latter, we demonstrated that the differences in a petal shape (pointed tip and rounded) could be measured by applying a semi-landmark method and the principal component analysis. We speculated that a petal shape could be a quantitative trait from our tetraploid population's segregation analysis of the petal shapes.
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| Free Research Field |
園芸作物を特徴付ける諸形質の組織化学的・遺伝学的・分子生物学的解析と農業生産への応用
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| Academic Significance and Societal Importance of the Research Achievements |
本課題では交雑集団を用いたアソシエーション解析によりトゲの有無と強い相関を示す染色体領域を見出しており，今後の研究の進展によりトゲ形成制御遺伝子の単離と機能解析を達成することができれば研究領域に非常に強いインパクトを与えることができると期待される．また，この染色体領域についてトゲの形成頻度が多い既存バラ品種と形成頻度が非常に少ない既存バラ品種を用いてDNA配列の比較解析を実施することで，トゲなしバラの効率的な育種に繋がるDNAマーカーの開発に繋がると期待される．
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