2022 Fiscal Year Final Research Report
Molecular mechanism of plant resilience from chilling stress via oil bodies
| Project/Area Number |
19K06039
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 39030:Horticultural science-related
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| Research Institution | Tokai University (2020-2022)
Niigata University (2019) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
伊藤 紀美子 新潟大学, 自然科学系, 教授 (10281007)
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | 花卉 / 休眠 / オイルボディ / リンドウ |
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| Outline of Final Research Achievements |
Gentian ortholog of OIL BODY-ASSOCIATED PROTEIN1 (GtOBAP1) was found as candidate gene involved in dormancy and budbreak of overwintering buds. The objective of this study is to elucidate the mechanism of dormancy regulation via oil bodies and GtOBAP1. In this study, we generated obap1 mutans by CRISPR/Cas9-mediated genome editing and found that the mutants showed suppression of growth recovery after the release of chilling stress, even though chilling injury was not observed. Furthermore, hydrolysis of triacylglycerols (TAGs) in oil bodies was suppressed in the obap1 mutants. These results indicated that GtOBAP1 may be involved in supply of oil body TAG-derived energy to resume growth after the release of chilling temperature.
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| Free Research Field |
植物代謝生理学
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| Academic Significance and Societal Importance of the Research Achievements |
植物におけるオイルボディの機能は大部分が未知であるが，本研究により成長回復や冬芽の萌芽に寄与する可能性が見出された．また，植物のストレス耐性がストレスで誘導される「抵抗力」と被ストレス状態から回復する「レジリエンス」の2段階で構成され，植物が安定したレジリエンスを得るためにオイルボディが中心的な役割を果たす可能性を示した．本成果は，植物のストレス耐性研究に新たな概念をもたらし，耐性強化育種に向けた新たなストラテジーとなることが期待できる．また，TAGは動植物共通の貯蔵脂質であることから，藻類のオイル産生や，動物のストレスレジリエンス等の研究への波及が期待できる．
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