Molecular mechanism of linalool mediated field resistance of citrus canker disease ( Xanthomonas citri subsp. citri)

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K06041
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39030:Horticultural science-related
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Shimada Takehiko 国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門, 主席研究員 (10355399)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: カンキツ / かいよう病 / 香気成分 / 圃場抵抗性

Outline of Final Research Achievements

It was clarified that linalool, an aromatic component accumulated in high content in the oil sac of leaves, contributes to the resistance to Ponkan citrus canker disease as an antibacterial substance. A negative correlation was observed between the linalool content in leaves and the number of citrus canker pathogens grown after inoculation. When QTL analysis was performed using the population of ``Harehime'' and ponkan, the QTL of both was in the same region of chromosome 3. It was suggested that citrus canker-resistant individuals could be efficiently acquired by selecting citrus plants that were mapped to the same region of citrus and contained linalool in the leaves at a level equal to or higher than that of ponkan.

Free Research Field

ゲノム育種

Academic Significance and Societal Importance of the Research Achievements

近年、温暖化の進行によりカンキツかいよう病の被害が深刻化し、抵抗性品種の開発が急務となっているが、ポンカンなどの圃場抵抗性育種素材のメカニズムは不明である。本研究により、ポンカンとヒュウガナツが示すカンキツかいよう病抵抗性に、葉の油胞組織にあらかじめ高含有に蓄積される香気成分リナロールが抗菌物質として寄与することが明らかとなった。ポンカンと同程度以上の量でリナロールを葉に含有したカンキツ個体を選抜することにより、カンキツかいよう病抵抗性個体を効率的に獲得することが期待され、カンキツかいよう病抵抗性育種の大幅な効率化に貢献する。