Clarification of ethylene-response mechanism using bud mutation in carnation.

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06043
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39030:Horticultural science-related
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: TANASE Koji 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門, グループ長補佐 (30355713)
• Co-Investigator(Kenkyū-buntansha): 小野崎 隆 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門, グループ長補佐 (90355719)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: カーネーション / 枝変わり / エチレン / 次世代シーケンサー

Outline of Final Research Achievements

In carnations, there are varieties with a reduced response to ethylene, which character is less susceptible to wilting in response to exogenous ethylene. We found a mutant with "normal ethylene response" red petals and "reduced ethylene response" orange petals within a single flower. We extracted RNA from the orange and red petals, respectively, and analyzed them by next-generation sequencing. Approximately 32,500 genes were identified for each petals. We extracted 503 genes whose expression levels differed more than 2-fold between red and orange petals. These genes include anthocyanin synthesis genes as well as transporters and other genes.

Free Research Field

園芸学

Academic Significance and Societal Importance of the Research Achievements

本研究で得られたデータを活用し、花弁の萎れ初期に起るインローリング（花弁が内側に巻き込む現象）が解明されると共に花持ち性育種が効率化される。これにより花持ち剤等の化学物質の低減や剤の処理による農家の労働時間の削減が可能となる。さらに鉢花などの花持ち剤の効果が低い品目の花持ち性が向上する。