Elucidation of the pathogenic mechanism of black spot bacterial disease using a comprehensive screening method

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06045
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: University of Tsukuba
• Principal Investigator: Ishiga Yasuhiro 筑波大学, 生命環境系, 助教 (50730256)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: アブラナ科植物黒斑細菌病 / キャベツ / 気孔防御

Outline of Final Research Achievements

We identified virulence genes related not only to type 3 secretion system (T3SS), but also to primary metabolisms including amino acids, lipids, carbohydrates and nucleic acids based on mutant screening of Pseudomonas cannabina pv. alisalensis. Furthermore, our results suggest that different virulence factors are required depending on the host. In addition, our results suggest that many virulence factors are involved in leaf surface growth and invasion.

Free Research Field

植物病理学

Academic Significance and Societal Importance of the Research Achievements

今回の研究成果から、アブラナ科植物黒斑細菌病菌の感染に必要な多くの病原力因子が葉の表面での増殖や侵入に関与することが示唆された。さらに、気孔の開閉をめぐる植物と病原細菌の攻防が感染の成否をめぐる重要な局面となっていることが示唆された。植物病原細菌の多くは気孔を侵入場所としており、さらに、いくつかの糸状菌も気孔を侵入場所としているため、気孔防御の活性化は、様々な植物病害防除への応用が可能になると考える。