2021 Fiscal Year Final Research Report
Studies on the regulation of callose deposition through MAMP-responsive phosphoprotein
Project/Area Number |
19K06053
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 39040:Plant protection science-related
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Research Institution | Okayama University |
Principal Investigator |
Matsui Hidenori 岡山大学, 環境生命科学学域, 准教授 (20598833)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | PTI / 植物免疫 / リン酸化 / カロース沈着 |
Outline of Final Research Achievements |
Understanding plant immunity is an important research topic for disease resistance breeding. When plants recognize pathogen infection, they activate an immune response to protect themselves from infection. Callose deposition at the site of infection is one of the plant immune responses and is thought to act as a physical barrier against pathogens. In this study, we aimed to elucidate the molecular mechanisms for the regulation of callose deposition by explaining the function of the mutant MARK2, which has reduced callose deposition. As MARK2 is a MAMP-responsive phosphoprotein, we also examined the significance of phosphorylation of MARK2. As a result, we found that phosphorylation of MARK2 protein contributes to the regulation of callose deposition during MAMP response. To understand the molecular function of MARK2, we successfully identified MARK2 interacting factors by co-immunoprecipitation.
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Free Research Field |
植物病理学
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Academic Significance and Societal Importance of the Research Achievements |
近年、環境負荷を低減した持続可能な農業の必要性が議論されている。環境負荷の低減には、作物への病害抵抗性の付与は欠かすことができない。そのためにも、植物が備える抵抗性の理解が必要である。本研究では、植物のMAMP認識を起点としたリン酸化シグナルの下流因子であるMARK2タンパク質の機能解明を通じて、植物が備える免疫応答の解明を進めた。その結果、MARK2タンパク質のリン酸化がカロース沈着制御に関わることを見出した。カロース沈着の制御プロセスの理解は学術的にも価値の高い成果であり、将来的にはカロース沈着の制御による物理的抵抗性の強化が可能になると期待される。
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