Identification of a nematode-resistant gene and its application to development of nematode-resistant cultivars in black oats

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K06064
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Naohiro Uwatoko 国立研究開発法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター, 主任研究員 (60582616)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: strigosaエンバク / サツマイモネコブセンチュウ抵抗性 / 線虫対抗品種 / 飼料作物 / 緑肥

Outline of Final Research Achievements

Analysis of an F2 population using GRAS-Di identified a 7.6cM region of the Mi locus that controls resistance to Meloidogyne incognita in black oats (Avena strigosa). All 53 RIL carrying the Mi locus region from 'Terara' showed resistance, and no other factors affecting Mi resistance were identified in the other region. A polymorphism survey using GRAS-Di was performed on 48 varieties and germplasm resources of black oats. It was shown that 'Terara' was genetically distant from the varieties distributed in Japan, and that the Mi locus region derived from 'Terara' could be detected in almost all cross combinations assumed for future breeding in Japan.

Free Research Field

植物遺伝育種学

Academic Significance and Societal Importance of the Research Achievements

strigosaエンバク「テララ」はサツマイモネコブセンチュウ（Mi）の増殖を抑制する稀有な線虫対抗品種であり、「テララ」の持つMi抵抗性はサツマイモネコブセンチュウ増殖リスクを回避できるため農業上の利用価値が高い。本研究の成果は、「テララ」のMi 抵抗性に関する遺伝的知見の理解を深めるとともに、線虫対抗エンバク品種の効率的な育成が可能になるMi抵抗性選抜マーカーの開発に資するものである。