2021 Fiscal Year Final Research Report
Developing long-term preservation method for hard-to-cryopreserve ectomycorrhizal basidiomycete cultures
| Project/Area Number |
19K06088
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 39060:Conservation of biological resources-related
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| Research Institution | Tottori University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
遠藤 直樹 鳥取大学, 農学部, 助教 (20776439)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 菌株保存 / 凍結保存 / 菌根性担子菌 / 難保存性培養株 / 菌根形成 / 多孔性培養基剤 |
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| Outline of Final Research Achievements |
This research aimed to develop and improve a useful cryopreservation method for cultures of mycorrhizal basidiomycetous fungi achieving high survival rates and maintaining hyphal growth rates and mycorrhiza forming ability in revived cultures. Our mycorrhiza synthesis method successfully generated artificial mycorrhizae on the root tips of Pinus densiflora seedlings and reproduced normal morphological characteristics identical to those of natural mycorrhizae, and the damage levels on fungal cultures caused by cryopreservation were assessed by the mycorrhiza formation rates on the root tips. Comparing and evaluating various preculturing methods for cryopreservation, we found "the Petri dish method" using vermiculites as culture substrate is more effective in achieving high survival rates and hyphal growth rates of revived cultures than the original "Tube method", whereas "the Agar disc method" is more efficient than "the Petri dish method" for maintaining mycorrhiza formation rates.
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| Free Research Field |
水界に生息する菌類の分類と生態に関する研究、および、菌株保存法の開発
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の成果は、生態学的にも経済的にも重要な菌群である菌根性担子菌類の培養株を長期間安定的に保存する方法の開発・改良に貢献するものである。これまで本菌群は分離・培養が難しいだけでなく、その培養株の保存が困難なために、世界の菌株保存機関でもわずかな菌株が保存されているのみで、入手が困難なため活用されることが少なかったが、菌株の性状を維持できる凍結保存法を開発することによって、様々な研究分野において本菌群の多種多様な培養株を生物資源として活用することが可能になる。
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