Accelerating floral surveys by introducing next-generation sequencers ~ a case study of the central highlands of Vietnam

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K06149
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 40010:Forest science-related
• Research Institution: Tokyo Gakugei University (2020-2022); Tokyo Metropolitan University (2019)
• Principal Investigator: OGURI Emiko 東京学芸大学, 教育学部, 研究員 (10608954)
• Co-Investigator(Kenkyū-buntansha): 田金 秀一郎 鹿児島大学, 総合研究博物館, 特任助教 (10616080)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 植物多様性 / 植物分類 / DNAバーコーディング / 分子系統 / ベトナム

Outline of Final Research Achievements

In this study, we conducted an exhaustive survey of the vascular flora in the Kon Chu Rang Nature Reserve and Kon Ka Kinh National Park in Gia Lai Province, central highlands, Vietnam, using a standardized belt-transect method and characterized the vascular flora of the study area by using DNA barcoding and morphological comparisons to identify and characterize species. Field surveys in the Kon Chu Rang Nature Reserve and Kon Ka Kinh National Park were conducted in August 2019 and December 2022, obtaining a total of 1,830 vascular plant specimens. For all specimens collected in August 2019, partial chloroplast rbcL sequences were obtained and species identification was performed by molecular identification and morphological comparison. As a result, new species were discovered and described from Lasianthus (Rubiaceae) and Leptochilus (Polypodiaceae), respectively for the flora of Kon Chu Rang Nature Reserve.

Free Research Field

植物系統分類学

Academic Significance and Societal Importance of the Research Achievements

本研究は、種同定をより正確かつ効率よく行うために葉緑体rbcLのDNAバーコーディングを活用している。本研究により、限られた人員、予算、時間の中で、迅速かつ正確に維管束植物相の解明のためのワークフローを構築・提案できる。それは、本研究の現地共同研究機関であるベトナム生態学生物資源研究所が、将来的に、独自にベトナム全土の植生調査を進めていく際の標準的なワークフローとなり得る。