2021 Fiscal Year Final Research Report
Characterization of the settlement inducing compound from conspecifics of the Pacific oyster Crassostrea gigas
| Project/Area Number |
19K06189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 40030:Aquatic bioproduction science-related
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
吉田 朝美 長崎大学, 水産・環境科学総合研究科(水産), 准教授 (80589870)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | マガキの付着誘起物質 / 貝殻マトリックスタンパク質 / Gigasin-6 X1/X2アイソフォーム / 付着フェロモン / 超分子相互作用 |
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| Outline of Final Research Achievements |
Gigasin-6 X1/X2, stains-all stainable acidic proteins (with a 48kDa major band) and surface protein P12p-like played synergistic roles in larval settlement induction of Crassostrea gigas through supramolecular action within the shell; a novel functional role for these shell matrix proteins (SMPs) as a settlement inducing cue. Sugar moieties of the settlement cue also influenced larval settlement, while endogenous ligands were also present in the larval chemoreceptors, and this may allow the larva greater selectivity during site selection. Genes related to shell formation showed closely linked dynamics with a gene regulatory network that may involve the interplay of various hormone receptors, neurotransmitters and neuropeptide receptors working together in a coordinated manner in both larval and post larval stages. Results also suggested a possible involvement of an ecdysone signaling pathway during C. gigas settlement.
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| Free Research Field |
ライフサイエンス／水圏生産科学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、いくつかのマガキゲノム由来のタンパク質配列に対して構造、機能及び生物学的注釈データを初めて示した。また、幼生の同種に対する識別について、付着誘起物質にある複数の糖鎖部位の関与が示され、今後の群居性の化学的根拠の解明に意義ある成果と考える。さらに、本種幼生の付着・変態は内分泌系伝達経路にバイオミネラリゼーション関連成分の影響が示された。本研究で解明された幼生と同種由来の付着誘起物質の相互作用は海産無脊椎動物幼生の付着機構研究において新しい知見である。本研究成果は、優良品種の高効率な採苗方法の開発に応用が期待される。
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