Biomarker exploration for evaluating egg quality of farmed fish by metabolome analysis

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K06190
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 40030:Aquatic bioproduction science-related
• Research Institution: University of Miyazaki
• Principal Investigator: Nagano Naoki 宮崎大学, 農学部, 教授 (50437943)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 養殖 / 種苗生産 / 卵質

Outline of Final Research Achievements

The purpose of this study is to search for effective biomarker candidates for evaluating egg quality by combining egg quality evaluation methods by breeding experiments and comprehensive analysis of metabolites existing in vivo. Fertilized eggs were collected from chub mackerel raised on different diets, and the hatching rates of fertilized eggs were compared. Fat-soluble and water-soluble components were extracted from the serum of the chub mackerel, and metabolites in the serum were searched. Finally, metabolite lists were compared between multiple samples and attempted to identify compounds that showed significant differences between the samples. As a result, the hatching rates of fertilized eggs differed depending on the fatty acid composition of the diet. Enrichment analysis of metabolic pathways of metabolites revealed differences in fatty acid metabolism.

Free Research Field

水産学

Academic Significance and Societal Importance of the Research Achievements

本研究では、マサバの親魚に給餌した飼料の脂肪酸組成および体内における脂肪酸代謝経路が受精卵の卵質に影響を及ぼすことが示唆された。マサバの卵質の評価方法は確立されておらず、本研究で行った卵質に影響を及ぼす因子の特定は、良質な受精卵を安定的に採取するための基礎知見となり、養殖用種苗の生産効率の向上に大きく貢献すると考えられる。