Analysis of genes for salt-tolerance in green foxtail toward breeding of salt-tolerant foxtail millet

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06341
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 41050:Environmental agriculture-related
• Research Institution: Kyoto Prefectural University
• Principal Investigator: Ohsako Takanori 京都府立大学, 生命環境科学研究科, 准教授 (80363969)
• Co-Investigator(Kenkyū-buntansha): 福永 健二 県立広島大学, 生命環境学部, 教授 (50435533)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: アワ / エノコログサ / 耐塩性 / QTL解析 / GRAS-Di / 適応進化

Outline of Final Research Achievements

We constructed linkage maps using two segregating populations derived from a cross between foxtail millet (74-12-6-3) and coastal green foxtail (Jogashima 1) and a cross between green foxtail (Katsurasaka 2) and coastal green foxtail (Mitsu 4) with GRAS-Di markers. Tolerance to salt stress was evaluated with F3 seedlings by hydroponic culture using media containing 100 mM and 0 mM (control) NaCl.
For 74-12-6-3 x Jogashima 1 population, a QTL for salt stress tolerance was detected on chromosome 5. For QTLs were seen on chromosomes 2, 3, 5, and 9 for Katsurasaka 2 x Mitsu 4 population. Our result indicates that geographically distinct populations possess different genetic architectures for salt stress tolerance and suggests parallel evolution of the traits for adaptation to coastal habitat.

Free Research Field

植物遺伝学

Academic Significance and Societal Importance of the Research Achievements

2系統のハマエノコロの耐塩性の遺伝的基盤が異なることが示され、国内のハマエノコロの耐塩性が平行進化により独立に獲得されたことが示唆された。この成果は海浜環境における植物の適応進化の解明に寄与するものである。
異なる野生種由来の複数の耐塩性関連遺伝子を交配などにより導入することで強力な耐塩性を持つ新規アワ系統を育成できる可能性が示され、塩性化土壌における作物生産の拡大に貢献する成果を得た。