2021 Fiscal Year Final Research Report
Study of the biological significance of oligosaccharide nucleotides separated from domestic farm animals' milk and attempt to utilize it in animal feed
| Project/Area Number |
19K06364
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42010:Animal production science-related
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
福田 健二 帯広畜産大学, 畜産学部, 教授 (80419217)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 糖ヌクレオチド / 酪農家畜 / 初乳 / 腸管バリア機能 / MUC2 / MUC3 / オリゴ糖ヌクレオチド |
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| Outline of Final Research Achievements |
Although the separation of oligosaccharide nucleotides from the colostra of domestic farm animals including, cow, goat and sheep, these were not separated. However, UDP-Gal, UDP-Glc, UDP-GalNAc and UDP-GlcNAc as sugar nucleotides were identified.
In in vitro experiment with intestinal epithelial cell, the growth stimulation had not been found by addition of UDP-Gal into the medium.To study the effect on intestinal barrier function by exposure with the sugar nucleotide, the mixed intestinal epithelial cell of Caco2:HT29-MTX cells =9 : 1, which was imitated for small intestine, was cultured with the final concentration of 200 μg/mL of UDP-Gal or UDP-Glc for 6, 12 or 24 hrs. Then total RNA was extracted from the cells and reverse - expressed to cDNA. The expressed levels of MUC2 or MUC3 was measured by real time PCR. As a result, the significant increase of the expression level for MUC2 was found in the cells, which were cultured with UDP-Gal for 6 hr.
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| Free Research Field |
糖質科学
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| Academic Significance and Societal Importance of the Research Achievements |
オリゴ糖ヌクレオチドという機能未知の物質の普遍的な存在と機能解明を行うことは、生化学に新たな知見を切り拓く壮大なチャレンジであったが達成できなかった。一方、酪農家畜の初乳に含まれる糖ヌクレオチドの種類を、従来の方法ではなく、現代的な精密な手法で明らかにしたことは意義がある。また、細胞内での糖鎖合成材料としてではなく、分泌液である初乳に含まれる糖ヌクレオチドが、乳子の腸管通過中にバリア機能に影響しうる可能性のあるデータがえられたことは、機能解明への今後の展開に希望をいだかせるものである。そのような試みによってその機能が解明されれば、家畜飼料・食品添加素材としての利用可能性をもたらすであろう。
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